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Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay

he biological solutions have become more familiar to remove hazardous substances from the environment. This present investigation was conducted in order to isolate fungi from contaminated soil collected from workshop area near Arau, Perlis. These isolated fungi were then tested on their ability to...

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Main Authors: Nabilah, Razak, Saiyidah Nafisah, Hashim, Chia, Chay Tay
Format: Conference or Workshop Item
Language:English
Published: 2015
Subjects:
Bioengineering
Environmental pollution
Online Access:http://ir.uitm.edu.my/id/eprint/33144/1/33144.pdf
http://ir.uitm.edu.my/id/eprint/33144/
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spelling my.uitm.ir.331442020-08-04T02:47:22Z http://ir.uitm.edu.my/id/eprint/33144/ Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay Nabilah, Razak Saiyidah Nafisah, Hashim Chia, Chay Tay Bioengineering Environmental pollution he biological solutions have become more familiar to remove hazardous substances from the environment. This present investigation was conducted in order to isolate fungi from contaminated soil collected from workshop area near Arau, Perlis. These isolated fungi were then tested on their ability to degrade petroleum oil on PDA medium that was polluted with different concentration of petroleum oil 2%,4% and 10% (v/v) and on the minimal salt solution by measuring changes in optical density (OD) read on spectrophotometer with 540 nm wavelength. Penicillium sp. was isolated from the contaminated soil using soil serial dilution method. Penicillium sp. was capable of actively degrade in varying degrees. The result showed significant difference (P<0.05) between different concentration of oil and the growth of fungi in 5 days of incubation period at 2% and 10% (v/v) petroleum oil concentration. Penicillium sp. showed highest growing diameter in 4% concentration of petroleum oil. Based on the optical density result, Penicillium sp. showed maximum peak of OD reading in day 20 with 0.66 ± 0.01, indicate that the highest ability of Penicillium sp. to degrade petroleum oil were in day 20. Statistical analysis of one-way ANOVA was done. The ability ofPenicillium sp. to tolerate oil pollutants and grow on them, suggest it can be employed as bioremediation agent and can be used in restoring contaminated ecosystem with oil. 2015 Conference or Workshop Item PeerReviewed text en http://ir.uitm.edu.my/id/eprint/33144/1/33144.pdf Nabilah, Razak and Saiyidah Nafisah, Hashim and Chia, Chay Tay (2015) Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay. In: Proceedings of Postgraduate Conference on Global Green Issues (Go Green), 7-8 October 2015, Dewan Kuliah Al-Khawarizmi Universiti Teknologi MARA Cawangan Perak.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bioengineering
Environmental pollution
spellingShingle Bioengineering
Environmental pollution
Nabilah, Razak
Saiyidah Nafisah, Hashim
Chia, Chay Tay
Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay
description he biological solutions have become more familiar to remove hazardous substances from the environment. This present investigation was conducted in order to isolate fungi from contaminated soil collected from workshop area near Arau, Perlis. These isolated fungi were then tested on their ability to degrade petroleum oil on PDA medium that was polluted with different concentration of petroleum oil 2%,4% and 10% (v/v) and on the minimal salt solution by measuring changes in optical density (OD) read on spectrophotometer with 540 nm wavelength. Penicillium sp. was isolated from the contaminated soil using soil serial dilution method. Penicillium sp. was capable of actively degrade in varying degrees. The result showed significant difference (P<0.05) between different concentration of oil and the growth of fungi in 5 days of incubation period at 2% and 10% (v/v) petroleum oil concentration. Penicillium sp. showed highest growing diameter in 4% concentration of petroleum oil. Based on the optical density result, Penicillium sp. showed maximum peak of OD reading in day 20 with 0.66 ± 0.01, indicate that the highest ability of Penicillium sp. to degrade petroleum oil were in day 20. Statistical analysis of one-way ANOVA was done. The ability ofPenicillium sp. to tolerate oil pollutants and grow on them, suggest it can be employed as bioremediation agent and can be used in restoring contaminated ecosystem with oil.
format Conference or Workshop Item
author Nabilah, Razak
Saiyidah Nafisah, Hashim
Chia, Chay Tay
author_facet Nabilah, Razak
Saiyidah Nafisah, Hashim
Chia, Chay Tay
author_sort Nabilah, Razak
title Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay
title_short Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay
title_full Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay
title_fullStr Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay
title_full_unstemmed Biodegradation of petroleum oil by using Isolated Penicillium sp. / Nabilah Razak, Saiyidah Nafisah Hashim and Chia Chay Tay
title_sort biodegradation of petroleum oil by using isolated penicillium sp. / nabilah razak, saiyidah nafisah hashim and chia chay tay
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/33144/1/33144.pdf
http://ir.uitm.edu.my/id/eprint/33144/
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score 13.211869

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