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Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]

Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family. The objectives of this study were to determine antimicrobial activity and Minimal Inhibition Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Met...

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Main Authors: Nawi, Liliwirianis, Md Isa, Nurun Nadhirah, Musa, Nor Lailatul Wahidah, Mohd Jan, Sarah Laila, Shaikh Nasir, Nur Thabitah
Format: Article
Language:English
Published: Universiti Teknologi MARA Cawangan Pahang 2019
Subjects:
Medicinal plants (Culture only)
Biotechnology
Online Access:http://ir.uitm.edu.my/id/eprint/31241/1/31241.pdf
http://ir.uitm.edu.my/id/eprint/31241/
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spelling my.uitm.ir.312412020-12-09T07:41:01Z http://ir.uitm.edu.my/id/eprint/31241/ Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.] Nawi, Liliwirianis Md Isa, Nurun Nadhirah Musa, Nor Lailatul Wahidah Mohd Jan, Sarah Laila Shaikh Nasir, Nur Thabitah Medicinal plants (Culture only) Biotechnology Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family. The objectives of this study were to determine antimicrobial activity and Minimal Inhibition Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Methanol and hexane were the two extraction solvents that had been used. Four concentrations of extracts; 50 mg/mL, 100 mg/mL, 200 mg/mL, and 400 mg/mL were prepared for antimicrobial activity. Meanwhile for MIC determination, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL and 50 mg/mL concentrations were prepared. By using disc diffusion method, the methanol extract of G. procumbens leaves showed antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa compared to hexane extract. Highest antimicrobial activities were recorded against S. aureus at 400 mg/mL concentrations with 10.5 mm of inhibition zone. Broth dilution assay resulted MIC for methanol crude extract against S. aureus, B. subtilis, K. pneumoniae, and P. aeruginosa were at 12.5 mg/mL, 25 mg/mL, and 50 mg/mL, respectively Universiti Teknologi MARA Cawangan Pahang 2019-03-31 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/31241/1/31241.pdf Nawi, Liliwirianis and Md Isa, Nurun Nadhirah and Musa, Nor Lailatul Wahidah and Mohd Jan, Sarah Laila and Shaikh Nasir, Nur Thabitah (2019) Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]. Gading Journal for Science and Technology, 2 (1). pp. 17-22. ISSN 2637-0018
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Medicinal plants (Culture only)
Biotechnology
spellingShingle Medicinal plants (Culture only)
Biotechnology
Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
description Gynura procumbens or Sambung Nyawa is a tropical plants species from the Asteraceae family. The objectives of this study were to determine antimicrobial activity and Minimal Inhibition Concentration (MIC) of G. procumbens leaf extracts against selective Gram-positive and Gramnegative bacteria. Methanol and hexane were the two extraction solvents that had been used. Four concentrations of extracts; 50 mg/mL, 100 mg/mL, 200 mg/mL, and 400 mg/mL were prepared for antimicrobial activity. Meanwhile for MIC determination, 6.25 mg/mL, 12.5 mg/mL, 25 mg/mL and 50 mg/mL concentrations were prepared. By using disc diffusion method, the methanol extract of G. procumbens leaves showed antimicrobial activities against Staphylococcus aureus, Bacillus subtilis, Klebsiella pneumoniae, and Pseudomonas aeruginosa compared to hexane extract. Highest antimicrobial activities were recorded against S. aureus at 400 mg/mL concentrations with 10.5 mm of inhibition zone. Broth dilution assay resulted MIC for methanol crude extract against S. aureus, B. subtilis, K. pneumoniae, and P. aeruginosa were at 12.5 mg/mL, 25 mg/mL, and 50 mg/mL, respectively
format Article
author Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
author_facet Nawi, Liliwirianis
Md Isa, Nurun Nadhirah
Musa, Nor Lailatul Wahidah
Mohd Jan, Sarah Laila
Shaikh Nasir, Nur Thabitah
author_sort Nawi, Liliwirianis
title Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_short Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_full Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_fullStr Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_full_unstemmed Antimicrobial activities of gynura procumbens leaves extract against selected bacteria / Liliwirianis Nawi ...[et al.]
title_sort antimicrobial activities of gynura procumbens leaves extract against selected bacteria / liliwirianis nawi ...[et al.]
publisher Universiti Teknologi MARA Cawangan Pahang
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/31241/1/31241.pdf
http://ir.uitm.edu.my/id/eprint/31241/
_version_ 1685650762312450048
score 13.211869

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