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Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir

The ornamental fish sector is a widespread and global component of international trade, fisheries, aquaculture and development. The utilization of multiple trade names causes problem in species identification. Moreover, unmanaged trading could lead to severe threats to biodiversity. In this regard,...

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Main Authors: Wan Zainal Azhar, Wan Fara Asyikin, Azmir, Izzati Adilah
Format: Article
Language:English
Published: Universiti Teknologi MARA, Negeri Sembilan 2019
Subjects:
Genetics
Fishes
Online Access:http://ir.uitm.edu.my/id/eprint/30589/1/30589.pdf
http://ir.uitm.edu.my/id/eprint/30589/
https://nsembilan.uitm.edu.my/joacns/
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spelling my.uitm.ir.305892020-06-01T01:56:15Z http://ir.uitm.edu.my/id/eprint/30589/ Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir Wan Zainal Azhar, Wan Fara Asyikin Azmir, Izzati Adilah Genetics Fishes The ornamental fish sector is a widespread and global component of international trade, fisheries, aquaculture and development. The utilization of multiple trade names causes problem in species identification. Moreover, unmanaged trading could lead to severe threats to biodiversity. In this regard, DNA barcoding could effectively clarify the divergence of the species. Considering the utility of DNA barcoding as a comprehensive system for species identification and discovery, this study aims to investigate the genetic relationship and to construct the phylogenetic tree among those selected fish species collected from selected pet stores in Seremban, Negeri Sembilan. The 642bp barcode fragment of the Cytochrome c oxidase I (COI) gene was PCR amplified. Results from BLAST showed all the generated sequence were subjected to high percentage identity index and similarity between 99% to 100%. It was then analyzed using MEGA 7.0 through Neighbour-Joining (NJ) clustering and K2P distance-based approach. The analysis revealed straightforward identification of eight specimens into five species with increasing value of genetic distances from conspecific (0.05%) to the taxonomic level (20.18%). The phylogenetic analysis consists of own sequences and reference sequences obtained from the GenBank. All the specimens from different genus was found with high bootstrap value (n>90%) through Neighbour-Joining (NJ) and Maximum Likelihood method. Thus, DNA barcoding reflects the efficacy of the techniques in identifying the genetic assessment in selected ornamental fishes. Universiti Teknologi MARA, Negeri Sembilan 2019 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/30589/1/30589.pdf Wan Zainal Azhar, Wan Fara Asyikin and Azmir, Izzati Adilah (2019) Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir. Journal of Academia, 7 (2). pp. 56-66. ISSN 2289-6368 https://nsembilan.uitm.edu.my/joacns/
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Genetics
Fishes
spellingShingle Genetics
Fishes
Wan Zainal Azhar, Wan Fara Asyikin
Azmir, Izzati Adilah
Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir
description The ornamental fish sector is a widespread and global component of international trade, fisheries, aquaculture and development. The utilization of multiple trade names causes problem in species identification. Moreover, unmanaged trading could lead to severe threats to biodiversity. In this regard, DNA barcoding could effectively clarify the divergence of the species. Considering the utility of DNA barcoding as a comprehensive system for species identification and discovery, this study aims to investigate the genetic relationship and to construct the phylogenetic tree among those selected fish species collected from selected pet stores in Seremban, Negeri Sembilan. The 642bp barcode fragment of the Cytochrome c oxidase I (COI) gene was PCR amplified. Results from BLAST showed all the generated sequence were subjected to high percentage identity index and similarity between 99% to 100%. It was then analyzed using MEGA 7.0 through Neighbour-Joining (NJ) clustering and K2P distance-based approach. The analysis revealed straightforward identification of eight specimens into five species with increasing value of genetic distances from conspecific (0.05%) to the taxonomic level (20.18%). The phylogenetic analysis consists of own sequences and reference sequences obtained from the GenBank. All the specimens from different genus was found with high bootstrap value (n>90%) through Neighbour-Joining (NJ) and Maximum Likelihood method. Thus, DNA barcoding reflects the efficacy of the techniques in identifying the genetic assessment in selected ornamental fishes.
format Article
author Wan Zainal Azhar, Wan Fara Asyikin
Azmir, Izzati Adilah
author_facet Wan Zainal Azhar, Wan Fara Asyikin
Azmir, Izzati Adilah
author_sort Wan Zainal Azhar, Wan Fara Asyikin
title Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir
title_short Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir
title_full Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir
title_fullStr Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir
title_full_unstemmed Genetic identification of selected ornamental fishes in Seremban, Negeri Sembilan / Wan Fara Asyikin Wan Zainal Azhar and Izzati Adilah Azmir
title_sort genetic identification of selected ornamental fishes in seremban, negeri sembilan / wan fara asyikin wan zainal azhar and izzati adilah azmir
publisher Universiti Teknologi MARA, Negeri Sembilan
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/30589/1/30589.pdf
http://ir.uitm.edu.my/id/eprint/30589/
https://nsembilan.uitm.edu.my/joacns/
_version_ 1685650678011133952
score 13.211869

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