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Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]

The aims of this study are to investigate antibacterial and antibiofilm activity of Rosa damascena (R.damascena) flower extract against Staphylococcus aureus (S. aureus). Method: The antibacterial activity was assessed with minimum inhibitory concentration (MIC) and minimum bactericidal concentratio...

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Main Authors: Mohd Amin, Indah, Mohamad Zain, Nurhayati, Ahmad, Vivi Noryati, Mat Daud, Dzulfakhrul Najwan, Abdullah Sani, Nabilah
Format: Conference or Workshop Item
Language:English
Published: 2019
Subjects:
Individual drugs and other agents
Materia medica
Online Access:http://ir.uitm.edu.my/id/eprint/30163/1/K_INDAH%20MOHD%20AMIN%20DSSS%20B%2019.pdf
http://ir.uitm.edu.my/id/eprint/30163/
https://dentistry.uitm.edu.my/v2/images/images/DSS/proceeding/vol1/pdf/12.pdf?
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spelling my.uitm.ir.301632020-04-23T02:52:12Z http://ir.uitm.edu.my/id/eprint/30163/ Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.] Mohd Amin, Indah Mohamad Zain, Nurhayati Ahmad, Vivi Noryati Mat Daud, Dzulfakhrul Najwan Abdullah Sani, Nabilah Individual drugs and other agents Materia medica The aims of this study are to investigate antibacterial and antibiofilm activity of Rosa damascena (R.damascena) flower extract against Staphylococcus aureus (S. aureus). Method: The antibacterial activity was assessed with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The potential of R.damascena flower extract as an antibiofilm agent was evaluated using biofilm inhibition assay and biofilm eradication assay. Chlorhexidine (CHX) 0.12% was used as a positive control. The experiment was performed in triplicate and repeated four times independently. All the data obtained were analysed using SPSS version 23. A Kruskal-Wallis test followed by post-hoc Mann-Whitney U test and level of significance was set at P < 0.05. Results: From this study, R.damascena extract shows antibacterial activity with MIC 50 mg/ml and MBC 100 mg/ml. Treatment with 0.5 MIC of extract inhibited 43.2% of biofilm formation. The biofilm eradication assay shows that 60.9% (p<0.001) of biofilm was eradicated by the extract upon 5 minutes treatment and 50.65% by 0.12% CHX. Conclusion: The result of this in-vitro study suggests a possible utilisation of R.damascena flower extract in treating S. aureus biofilm-associated infection in the oral environment. 2019 Conference or Workshop Item PeerReviewed text en http://ir.uitm.edu.my/id/eprint/30163/1/K_INDAH%20MOHD%20AMIN%20DSSS%20B%2019.pdf Mohd Amin, Indah and Mohamad Zain, Nurhayati and Ahmad, Vivi Noryati and Mat Daud, Dzulfakhrul Najwan and Abdullah Sani, Nabilah (2019) Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]. In: Proceedings of 9th Dental Student’s Scientific Symposium,Malaysia (Malaysia, 2019), 9th – 10th April 2019, Faculty Dentistry, Universiti Teknologi MARA. https://dentistry.uitm.edu.my/v2/images/images/DSS/proceeding/vol1/pdf/12.pdf?
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Individual drugs and other agents
Materia medica
spellingShingle Individual drugs and other agents
Materia medica
Mohd Amin, Indah
Mohamad Zain, Nurhayati
Ahmad, Vivi Noryati
Mat Daud, Dzulfakhrul Najwan
Abdullah Sani, Nabilah
Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]
description The aims of this study are to investigate antibacterial and antibiofilm activity of Rosa damascena (R.damascena) flower extract against Staphylococcus aureus (S. aureus). Method: The antibacterial activity was assessed with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The potential of R.damascena flower extract as an antibiofilm agent was evaluated using biofilm inhibition assay and biofilm eradication assay. Chlorhexidine (CHX) 0.12% was used as a positive control. The experiment was performed in triplicate and repeated four times independently. All the data obtained were analysed using SPSS version 23. A Kruskal-Wallis test followed by post-hoc Mann-Whitney U test and level of significance was set at P < 0.05. Results: From this study, R.damascena extract shows antibacterial activity with MIC 50 mg/ml and MBC 100 mg/ml. Treatment with 0.5 MIC of extract inhibited 43.2% of biofilm formation. The biofilm eradication assay shows that 60.9% (p<0.001) of biofilm was eradicated by the extract upon 5 minutes treatment and 50.65% by 0.12% CHX. Conclusion: The result of this in-vitro study suggests a possible utilisation of R.damascena flower extract in treating S. aureus biofilm-associated infection in the oral environment.
format Conference or Workshop Item
author Mohd Amin, Indah
Mohamad Zain, Nurhayati
Ahmad, Vivi Noryati
Mat Daud, Dzulfakhrul Najwan
Abdullah Sani, Nabilah
author_facet Mohd Amin, Indah
Mohamad Zain, Nurhayati
Ahmad, Vivi Noryati
Mat Daud, Dzulfakhrul Najwan
Abdullah Sani, Nabilah
author_sort Mohd Amin, Indah
title Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]
title_short Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]
title_full Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]
title_fullStr Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]
title_full_unstemmed Antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / Indah Mohd Amin … [et al.]
title_sort antimicrobial and antibiofilm activities of the methanol extracts of rosa damascena against s. aureus / indah mohd amin … [et al.]
publishDate 2019
url http://ir.uitm.edu.my/id/eprint/30163/1/K_INDAH%20MOHD%20AMIN%20DSSS%20B%2019.pdf
http://ir.uitm.edu.my/id/eprint/30163/
https://dentistry.uitm.edu.my/v2/images/images/DSS/proceeding/vol1/pdf/12.pdf?
_version_ 1685650617298583552
score 13.211869

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