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Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri

Polygonum minus, subspecies of a large medicinal genus of Polygonaceae have long provided mankind with its tremendous benefits in providing ailment for various illnesses. Despite its aromatic flavors, P. minus had been reported to possess high polyphenol content that responsible for its antibacteria...

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Main Author: Mohd Zamri, Nurul Alia Farhah
Format: Thesis
Language:English
Published: 2015
Subjects:
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/28098/1/TD_NURUL%20ALIA%20FARHAH%20MOHD%20ZAMRI%20HS%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/28098/
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spelling my.uitm.ir.280982020-02-03T03:42:52Z http://ir.uitm.edu.my/id/eprint/28098/ Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri Mohd Zamri, Nurul Alia Farhah Bacteria Polygonum minus, subspecies of a large medicinal genus of Polygonaceae have long provided mankind with its tremendous benefits in providing ailment for various illnesses. Despite its aromatic flavors, P. minus had been reported to possess high polyphenol content that responsible for its antibacterial and antioxidant activities. P. minus had been traditionally used to treat gastrointestinal related diseases which this property can be the answer to resolve emerging elevated multi-resistant in foodborne pathogens. This study attempts to evaluate total phenolic content and antibacterial activity of P. minus leaves methanolic extract against foodborne pathogens which are Staphylococcus aureus (ATCC 43300), Bacillus cereus (ATCC 14579), Escherechia coli (ATCC 25922) and Salmonella typhimurium (ATCC 13311). In this study, polyphenol contained in P. minus extract analyzed through Folin-Ciocalteu assay presented by 11.67±0.77 GAE/g. Screening of antibacterial activity was performed using disc diffusion method at two concentrations of P. minus extract (250mg/ml and 500mg/ml). The preliminary screening of inhibition zone depicts that the extracts were more susceptible towards gram positive bacteria contrast to gram negative bacteria. Staphylococcus aureus and Bacillus cereus were subjected to Minumum Inhibitory Concentration (MIC) using a microbroth dilution method since both bacteria showed presence of inhibition zone in the initial screening test. The most striking result is that both bacteria activity were inhibited at 31.25mg/ml P. minus extract. Whereas the Minimum Bactericidal Concentration (MBC) of P. minus methanolic extract was 3.91 mg/m!. Thus, this study proved that P. minus methanolic extract has emerged as an alternative for microbial growth inhibitor in foods and its significant tremendous total phenolic content has potential as a natural antioxidant agent. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/28098/1/TD_NURUL%20ALIA%20FARHAH%20MOHD%20ZAMRI%20HS%2015_5.pdf Mohd Zamri, Nurul Alia Farhah (2015) Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri. Degree thesis, Universiti Teknologi MARA.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bacteria
spellingShingle Bacteria
Mohd Zamri, Nurul Alia Farhah
Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri
description Polygonum minus, subspecies of a large medicinal genus of Polygonaceae have long provided mankind with its tremendous benefits in providing ailment for various illnesses. Despite its aromatic flavors, P. minus had been reported to possess high polyphenol content that responsible for its antibacterial and antioxidant activities. P. minus had been traditionally used to treat gastrointestinal related diseases which this property can be the answer to resolve emerging elevated multi-resistant in foodborne pathogens. This study attempts to evaluate total phenolic content and antibacterial activity of P. minus leaves methanolic extract against foodborne pathogens which are Staphylococcus aureus (ATCC 43300), Bacillus cereus (ATCC 14579), Escherechia coli (ATCC 25922) and Salmonella typhimurium (ATCC 13311). In this study, polyphenol contained in P. minus extract analyzed through Folin-Ciocalteu assay presented by 11.67±0.77 GAE/g. Screening of antibacterial activity was performed using disc diffusion method at two concentrations of P. minus extract (250mg/ml and 500mg/ml). The preliminary screening of inhibition zone depicts that the extracts were more susceptible towards gram positive bacteria contrast to gram negative bacteria. Staphylococcus aureus and Bacillus cereus were subjected to Minumum Inhibitory Concentration (MIC) using a microbroth dilution method since both bacteria showed presence of inhibition zone in the initial screening test. The most striking result is that both bacteria activity were inhibited at 31.25mg/ml P. minus extract. Whereas the Minimum Bactericidal Concentration (MBC) of P. minus methanolic extract was 3.91 mg/m!. Thus, this study proved that P. minus methanolic extract has emerged as an alternative for microbial growth inhibitor in foods and its significant tremendous total phenolic content has potential as a natural antioxidant agent.
format Thesis
author Mohd Zamri, Nurul Alia Farhah
author_facet Mohd Zamri, Nurul Alia Farhah
author_sort Mohd Zamri, Nurul Alia Farhah
title Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri
title_short Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri
title_full Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri
title_fullStr Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri
title_full_unstemmed Evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / Nurul Alia Farhah Mohd Zamri
title_sort evaluation of total phenolic content and antibacterial activity of palygnnum minus leaves methanolic extract against food borne pathogens / nurul alia farhah mohd zamri
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/28098/1/TD_NURUL%20ALIA%20FARHAH%20MOHD%20ZAMRI%20HS%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/28098/
_version_ 1685650365628809216
score 13.211869

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