Cover Image

Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria

Citrus grandis flowers possesses a strong floral-, jasmine - and orange- like aroma that has a potential in perfumery industries. Three extraction techniques that is hydrodistillation, Soxhlet extraction and solid phase micro extraction (SPME) were coupling with gas chromatograph -mass spectrometry...

Full description

Saved in:
Bibliographic Details
Main Author: Zakaria, Suhana
Format: Thesis
Language:English
Published: 2009
Subjects:
Plant anatomy
Plant physiology
Online Access:http://ir.uitm.edu.my/id/eprint/27593/1/TM_SUHANA%20ZAKARIA%20AS%2009_5.pdf
http://ir.uitm.edu.my/id/eprint/27593/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.uitm.ir.27593
record_format eprints
spelling my.uitm.ir.275932020-01-22T05:35:20Z http://ir.uitm.edu.my/id/eprint/27593/ Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria Zakaria, Suhana Plant anatomy Plant physiology Citrus grandis flowers possesses a strong floral-, jasmine - and orange- like aroma that has a potential in perfumery industries. Three extraction techniques that is hydrodistillation, Soxhlet extraction and solid phase micro extraction (SPME) were coupling with gas chromatograph -mass spectrometry (GC-MS) for analysis of fragrance compounds from this flowers. About 120 compounds were detected in bud and blossom of these flowers, of which the five major fragrance compounds were β-myrcene, limonene, ocimene, linalool and caryophyllene. In hydrodistillation, ocimene and linalool detected in blossom were higher than in bud with 4.57%. However, Soxhlet extraction showed that bud contained high percent of limonene (27.25%), ocimene (5.55%) and linalool (2.97%) compared to blossom. Three different SPME fibers were used in this study, namely, 65 µm CAR/PDMS, 75 µm PDMS/DVB and 100 µm PDMS. The best result was obtained by 100 µM PDMS, that were then further analyzed for optimum sampling time. It showed 60 min fiber exposition time was the optimum sampling time to extract the major fragrance compounds from the headspace. In cell culture development, callus was successfully induced from different part of C. grandis flowers like petal , sepal, style, ovary, pistil and cup base on Murashige and Skoog (MS) medium supplemented with sucrose (30 g/l) and various concentrations of hormones. It was found that different parts of flowers required different level of hormone for callus induction. The highest formation of callus were obtained from petal and sepal cultured on MS media supplemented with 1.5 mg/l kinetin. For the style and pistil, 50% of the explants had developed callus when cultured on MS media added with 0.05 mg/l and 0.10 mg/l BAP respectively. On the other hand, sucrose agar media alone managed to induce callus formation from the cup base and ovary with success rate between 11 ± 1.29 to 25 ± 4.19%. Callus obtained were then subjected to SPME for determination of compounds in the callus. Seven compounds were detected in the callus which some of the compounds shows the same compounds as detected in the fresh flowers. 2009 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/27593/1/TM_SUHANA%20ZAKARIA%20AS%2009_5.pdf Zakaria, Suhana (2009) Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria. Masters thesis, Universiti Teknologi MARA.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Plant anatomy
Plant physiology
spellingShingle Plant anatomy
Plant physiology
Zakaria, Suhana
Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
description Citrus grandis flowers possesses a strong floral-, jasmine - and orange- like aroma that has a potential in perfumery industries. Three extraction techniques that is hydrodistillation, Soxhlet extraction and solid phase micro extraction (SPME) were coupling with gas chromatograph -mass spectrometry (GC-MS) for analysis of fragrance compounds from this flowers. About 120 compounds were detected in bud and blossom of these flowers, of which the five major fragrance compounds were β-myrcene, limonene, ocimene, linalool and caryophyllene. In hydrodistillation, ocimene and linalool detected in blossom were higher than in bud with 4.57%. However, Soxhlet extraction showed that bud contained high percent of limonene (27.25%), ocimene (5.55%) and linalool (2.97%) compared to blossom. Three different SPME fibers were used in this study, namely, 65 µm CAR/PDMS, 75 µm PDMS/DVB and 100 µm PDMS. The best result was obtained by 100 µM PDMS, that were then further analyzed for optimum sampling time. It showed 60 min fiber exposition time was the optimum sampling time to extract the major fragrance compounds from the headspace. In cell culture development, callus was successfully induced from different part of C. grandis flowers like petal , sepal, style, ovary, pistil and cup base on Murashige and Skoog (MS) medium supplemented with sucrose (30 g/l) and various concentrations of hormones. It was found that different parts of flowers required different level of hormone for callus induction. The highest formation of callus were obtained from petal and sepal cultured on MS media supplemented with 1.5 mg/l kinetin. For the style and pistil, 50% of the explants had developed callus when cultured on MS media added with 0.05 mg/l and 0.10 mg/l BAP respectively. On the other hand, sucrose agar media alone managed to induce callus formation from the cup base and ovary with success rate between 11 ± 1.29 to 25 ± 4.19%. Callus obtained were then subjected to SPME for determination of compounds in the callus. Seven compounds were detected in the callus which some of the compounds shows the same compounds as detected in the fresh flowers.
format Thesis
author Zakaria, Suhana
author_facet Zakaria, Suhana
author_sort Zakaria, Suhana
title Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_short Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_full Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_fullStr Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_full_unstemmed Compounds and development of cell cultures of Citrus grandis (OSBECK.) flowers / Suhana Zakaria
title_sort compounds and development of cell cultures of citrus grandis (osbeck.) flowers / suhana zakaria
publishDate 2009
url http://ir.uitm.edu.my/id/eprint/27593/1/TM_SUHANA%20ZAKARIA%20AS%2009_5.pdf
http://ir.uitm.edu.my/id/eprint/27593/
_version_ 1685650294854123520
score 13.211869

Similar Items