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Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar

The simple method Single Drop Microextraction and High Performance Liquid Chromatography has been developed for analysis of two different pesticides; methidathion and vinclozolin. The effect of mobile phase composition and flow rate of pump has been studied to determine the separation efficiency of...

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Main Author: Hairul Anuar, Syaza Fakhriah
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2016
Subjects:
QD Chemistry
Fluorine
Online Access:http://ir.uitm.edu.my/id/eprint/24296/1/PPb_SYAZA%20FAKHRIAH%20HAIRUL%20ANUAR%20AS%20N%2016_5.pdf
http://ir.uitm.edu.my/id/eprint/24296/
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spelling my.uitm.ir.242962019-05-23T07:59:51Z http://ir.uitm.edu.my/id/eprint/24296/ Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar Hairul Anuar, Syaza Fakhriah QD Chemistry Fluorine The simple method Single Drop Microextraction and High Performance Liquid Chromatography has been developed for analysis of two different pesticides; methidathion and vinclozolin. The effect of mobile phase composition and flow rate of pump has been studied to determine the separation efficiency of analytes. Two different mobile phase composition of acetonitrile-water of 55:45 and 70:30 (v/v) ratios with flow rate of 0.8 mL/min and 1.0 mL/min respectively were studied in order to determine the best parameter for the developed method. The best experimental conditions of the proposed HPLC method were 10 μL injection volumes, mobile phase acetonitrile-water with composition 55:45 (v/v) as eluent at flow rate of 0.8 mL/min. The extraction time of SDME technique was studied and optimized while the other parameters such as organic solvent, stirring rate, droplet volume and volume of aqueous sample were constant during the study. The extraction time was varied in the range of 10 to 30 minutes with 5 minutes interval. The separation of extracted samples was achieved by injected 1 μL of sample in HPLC in order to reduce peak broadening. The optimized parameter of SDME technique in this study were 25 minutes of extraction time, toluene as organic solvent, 200 rpm stirring rate, 3 μL microdrop volume and 4 mL aqueous samples. The SDME technique with HPLC-UV analysis has provided a simple, reliable, reproducible and low in cost for analysis of pesticides. Faculty of Applied Sciences 2016 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24296/1/PPb_SYAZA%20FAKHRIAH%20HAIRUL%20ANUAR%20AS%20N%2016_5.pdf Hairul Anuar, Syaza Fakhriah (2016) Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QD Chemistry
Fluorine
spellingShingle QD Chemistry
Fluorine
Hairul Anuar, Syaza Fakhriah
Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar
description The simple method Single Drop Microextraction and High Performance Liquid Chromatography has been developed for analysis of two different pesticides; methidathion and vinclozolin. The effect of mobile phase composition and flow rate of pump has been studied to determine the separation efficiency of analytes. Two different mobile phase composition of acetonitrile-water of 55:45 and 70:30 (v/v) ratios with flow rate of 0.8 mL/min and 1.0 mL/min respectively were studied in order to determine the best parameter for the developed method. The best experimental conditions of the proposed HPLC method were 10 μL injection volumes, mobile phase acetonitrile-water with composition 55:45 (v/v) as eluent at flow rate of 0.8 mL/min. The extraction time of SDME technique was studied and optimized while the other parameters such as organic solvent, stirring rate, droplet volume and volume of aqueous sample were constant during the study. The extraction time was varied in the range of 10 to 30 minutes with 5 minutes interval. The separation of extracted samples was achieved by injected 1 μL of sample in HPLC in order to reduce peak broadening. The optimized parameter of SDME technique in this study were 25 minutes of extraction time, toluene as organic solvent, 200 rpm stirring rate, 3 μL microdrop volume and 4 mL aqueous samples. The SDME technique with HPLC-UV analysis has provided a simple, reliable, reproducible and low in cost for analysis of pesticides.
format Student Project
author Hairul Anuar, Syaza Fakhriah
author_facet Hairul Anuar, Syaza Fakhriah
author_sort Hairul Anuar, Syaza Fakhriah
title Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar
title_short Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar
title_full Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar
title_fullStr Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar
title_full_unstemmed Method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / Syaza Fakhriah Hairul Anuar
title_sort method development of single drop microextraction for pesticides analysis using high performance liquid chromatography / syaza fakhriah hairul anuar
publisher Faculty of Applied Sciences
publishDate 2016
url http://ir.uitm.edu.my/id/eprint/24296/1/PPb_SYAZA%20FAKHRIAH%20HAIRUL%20ANUAR%20AS%20N%2016_5.pdf
http://ir.uitm.edu.my/id/eprint/24296/
_version_ 1685649839204859904
score 13.211869

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