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Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan

Lead is metal that has very high toxicity and dangerous to human body. Once it had been absorbed in the body, it would deposit on vital organ such as brain and induce apoptosis, a process of cell death. This study aimed to examine the effect of lead toxicity on apoptosis of brain cells through light...

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Main Author: Mhd Ngadisan, Syasya Aqilah
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2015
Subjects:
QR Microbiology
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/24080/1/PPb_SYASYA%20AQILAH%20MHD%20NGADISAN%20AS%20N%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/24080/
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spelling my.uitm.ir.240802019-05-08T01:07:14Z http://ir.uitm.edu.my/id/eprint/24080/ Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan Mhd Ngadisan, Syasya Aqilah QR Microbiology Bacteria Lead is metal that has very high toxicity and dangerous to human body. Once it had been absorbed in the body, it would deposit on vital organ such as brain and induce apoptosis, a process of cell death. This study aimed to examine the effect of lead toxicity on apoptosis of brain cells through light microscopic examinations and at the same time, this study aimed to determine the morphological differences between normal cells and death cells. The brains were harvested from two rat groups, controlled group and lead-treated group. The rats in control group were fed with distilled water while rats of treated group were fed with distilled water and 0.2% lead acetate. 0.2% lead acetate was used in this study because previous research recorded that low level of lead exposure could induce cell apoptosis and may cause impairments in learning, memory and neurologic development especially in rats. All these brain samples were obtained from Dentistry Department, Uitm Shah Alam. The brains were sliced into extremely thin sections using microtome and stained using haematoxylin and eosin (H&E). The H&E staining is a staining method that had been widely used in histology to determine the structure of the cells. The haematoxylin stained the nucleus blue while eosin stained the cytoplasm with red colour. The result of this study demonstrated that low level of lead exposure could induce apoptosis in brain cells and it proved that H&E staining method is successful and reliable to observe and differentiate between normal and death cells. For future research, it is recommended to study the expression of genes that control the cell apoptosis for more precise and accurate assessment. The addition of more parameters helps to justify the validity of the results. Faculty of Applied Sciences 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/24080/1/PPb_SYASYA%20AQILAH%20MHD%20NGADISAN%20AS%20N%2015_5.pdf Mhd Ngadisan, Syasya Aqilah (2015) Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan. [Student Project] (Submitted)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QR Microbiology
Bacteria
spellingShingle QR Microbiology
Bacteria
Mhd Ngadisan, Syasya Aqilah
Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
description Lead is metal that has very high toxicity and dangerous to human body. Once it had been absorbed in the body, it would deposit on vital organ such as brain and induce apoptosis, a process of cell death. This study aimed to examine the effect of lead toxicity on apoptosis of brain cells through light microscopic examinations and at the same time, this study aimed to determine the morphological differences between normal cells and death cells. The brains were harvested from two rat groups, controlled group and lead-treated group. The rats in control group were fed with distilled water while rats of treated group were fed with distilled water and 0.2% lead acetate. 0.2% lead acetate was used in this study because previous research recorded that low level of lead exposure could induce cell apoptosis and may cause impairments in learning, memory and neurologic development especially in rats. All these brain samples were obtained from Dentistry Department, Uitm Shah Alam. The brains were sliced into extremely thin sections using microtome and stained using haematoxylin and eosin (H&E). The H&E staining is a staining method that had been widely used in histology to determine the structure of the cells. The haematoxylin stained the nucleus blue while eosin stained the cytoplasm with red colour. The result of this study demonstrated that low level of lead exposure could induce apoptosis in brain cells and it proved that H&E staining method is successful and reliable to observe and differentiate between normal and death cells. For future research, it is recommended to study the expression of genes that control the cell apoptosis for more precise and accurate assessment. The addition of more parameters helps to justify the validity of the results.
format Student Project
author Mhd Ngadisan, Syasya Aqilah
author_facet Mhd Ngadisan, Syasya Aqilah
author_sort Mhd Ngadisan, Syasya Aqilah
title Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_short Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_full Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_fullStr Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_full_unstemmed Histological study following lead exposure of sprague dawley brain / Syasya Aqilah Mhd Ngadisan
title_sort histological study following lead exposure of sprague dawley brain / syasya aqilah mhd ngadisan
publisher Faculty of Applied Sciences
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/24080/1/PPb_SYASYA%20AQILAH%20MHD%20NGADISAN%20AS%20N%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/24080/
_version_ 1685649809768185856
score 13.211869

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