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Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail

Melastoma malabathricum is a plant that has benefits in medicine. All parts of M malabathricum from root to shoot have potential to treat the diseases. The objectives of the study are to perform sequential extraction, to identify the secondary metabolites and to determine the antimicrobial activi...

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Main Author: Wan Ismail, Wan Nor Hamizah
Format: Student Project
Language:English
Published: Faculty of Applied Sciences 2017
Subjects:
Bacteria
Online Access:http://ir.uitm.edu.my/id/eprint/22900/1/PPb_WAN%20NOR%20HAMIZAH%20WAN%20ISMAIL%20AS%20C%2017_5.PDF
http://ir.uitm.edu.my/id/eprint/22900/
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spelling my.uitm.ir.229002019-01-23T01:23:51Z http://ir.uitm.edu.my/id/eprint/22900/ Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail Wan Ismail, Wan Nor Hamizah Bacteria Melastoma malabathricum is a plant that has benefits in medicine. All parts of M malabathricum from root to shoot have potential to treat the diseases. The objectives of the study are to perform sequential extraction, to identify the secondary metabolites and to determine the antimicrobial activities against two bacteria which are Staphylococcus aureus and Pseudomonas aeruginosa. The solvents used for phytochemical screening test were hexane and methanol. Antimicrobial activity was investigated using disk diffusion method. The extracts were test with different concentration which 100 mg/ml, 80 mg/ml, 60 mg/ml, 40 mg/ml, and 20 mg/ml. The phytochemical analysis of hexane extracts showed the presence of flavonoid, phenol and tannins, while methanol extracts showed the presence of glycoside and tannins. Different concentrations of crude extract show significant difference (P ≤ 0.05). Methanol leaves extract at concentration of 100 mg/ml showed the highest antibacterial activity against S. aureus and P. aeruginosa with zone of inhibition of 16 mm and 26 mm respectively. Thus, it indicate that methanol leaves extract contain high components related to antibacterial activity. As a conclusion M malabathricum methanol leaves extract potentially can be used against gram positive and negative bacteria. Faculty of Applied Sciences 2017 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/22900/1/PPb_WAN%20NOR%20HAMIZAH%20WAN%20ISMAIL%20AS%20C%2017_5.PDF Wan Ismail, Wan Nor Hamizah (2017) Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bacteria
spellingShingle Bacteria
Wan Ismail, Wan Nor Hamizah
Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail
description Melastoma malabathricum is a plant that has benefits in medicine. All parts of M malabathricum from root to shoot have potential to treat the diseases. The objectives of the study are to perform sequential extraction, to identify the secondary metabolites and to determine the antimicrobial activities against two bacteria which are Staphylococcus aureus and Pseudomonas aeruginosa. The solvents used for phytochemical screening test were hexane and methanol. Antimicrobial activity was investigated using disk diffusion method. The extracts were test with different concentration which 100 mg/ml, 80 mg/ml, 60 mg/ml, 40 mg/ml, and 20 mg/ml. The phytochemical analysis of hexane extracts showed the presence of flavonoid, phenol and tannins, while methanol extracts showed the presence of glycoside and tannins. Different concentrations of crude extract show significant difference (P ≤ 0.05). Methanol leaves extract at concentration of 100 mg/ml showed the highest antibacterial activity against S. aureus and P. aeruginosa with zone of inhibition of 16 mm and 26 mm respectively. Thus, it indicate that methanol leaves extract contain high components related to antibacterial activity. As a conclusion M malabathricum methanol leaves extract potentially can be used against gram positive and negative bacteria.
format Student Project
author Wan Ismail, Wan Nor Hamizah
author_facet Wan Ismail, Wan Nor Hamizah
author_sort Wan Ismail, Wan Nor Hamizah
title Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail
title_short Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail
title_full Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail
title_fullStr Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail
title_full_unstemmed Screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / Wan Nor Hamizah Wan Ismail
title_sort screening for antmacterial activities of melastoma malabathricum leaves extract against selected microorganism / wan nor hamizah wan ismail
publisher Faculty of Applied Sciences
publishDate 2017
url http://ir.uitm.edu.my/id/eprint/22900/1/PPb_WAN%20NOR%20HAMIZAH%20WAN%20ISMAIL%20AS%20C%2017_5.PDF
http://ir.uitm.edu.my/id/eprint/22900/
_version_ 1685649666614493184
score 13.211869

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