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Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz

Lignin is a phenolic heteropolymer in secondary cell walls that plays a main role in the development of plants and defence against any pathogens. The biosynthesis of monolignols, which represents the important component of lignin involves large amount enzymes. The Cinnamyl Alcohol Dehydrogenase (CAD...

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Main Author: Abdul Aziz, Nur Afiqah
Format: Student Project
Language:English
Published: Faculty of Plantation and Agrotechnology 2018
Subjects:
S Agriculture (General)
Agriculture and the environment
Online Access:http://ir.uitm.edu.my/id/eprint/22769/1/22769.pdf
http://ir.uitm.edu.my/id/eprint/22769/
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spelling my.uitm.ir.227692020-08-04T02:49:21Z http://ir.uitm.edu.my/id/eprint/22769/ Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz Abdul Aziz, Nur Afiqah S Agriculture (General) Agriculture and the environment Lignin is a phenolic heteropolymer in secondary cell walls that plays a main role in the development of plants and defence against any pathogens. The biosynthesis of monolignols, which represents the important component of lignin involves large amount enzymes. The Cinnamyl Alcohol Dehydrogenase (CAD) is a key enzyme in biosynthesis of lignin that catalyses the final step in the biosynthesis of monolignols. Based on previous sequence analysis, EgCAD1 is a bona fide CAD gene involves in lignin biosynthesis in oil palm. However, it is not supported by any scientific evidences. Functional study of the gene is required to prove the statement. Therefore, this study was performed to develop transgenic plants carrying the EgCAD1 gene from oil palm in order to provide materials for future functional study of the gene. Agrobacterium-mediated transformation method was applied in this research to transform the tobacco leaf discs. Shoots were regenerated from the Agrobacterium-infected leaf discs. Subsequently, the shoots were subcultured on rooting medium to induce root formation. Multiple lines of transgenic tobacco plants were produced through this study. The transgenic plants developed in this study will permit one to identify the functions of EgCAD1 gene in future. Faculty of Plantation and Agrotechnology 2018 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/22769/1/22769.pdf Abdul Aziz, Nur Afiqah (2018) Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic S Agriculture (General)
Agriculture and the environment
spellingShingle S Agriculture (General)
Agriculture and the environment
Abdul Aziz, Nur Afiqah
Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
description Lignin is a phenolic heteropolymer in secondary cell walls that plays a main role in the development of plants and defence against any pathogens. The biosynthesis of monolignols, which represents the important component of lignin involves large amount enzymes. The Cinnamyl Alcohol Dehydrogenase (CAD) is a key enzyme in biosynthesis of lignin that catalyses the final step in the biosynthesis of monolignols. Based on previous sequence analysis, EgCAD1 is a bona fide CAD gene involves in lignin biosynthesis in oil palm. However, it is not supported by any scientific evidences. Functional study of the gene is required to prove the statement. Therefore, this study was performed to develop transgenic plants carrying the EgCAD1 gene from oil palm in order to provide materials for future functional study of the gene. Agrobacterium-mediated transformation method was applied in this research to transform the tobacco leaf discs. Shoots were regenerated from the Agrobacterium-infected leaf discs. Subsequently, the shoots were subcultured on rooting medium to induce root formation. Multiple lines of transgenic tobacco plants were produced through this study. The transgenic plants developed in this study will permit one to identify the functions of EgCAD1 gene in future.
format Student Project
author Abdul Aziz, Nur Afiqah
author_facet Abdul Aziz, Nur Afiqah
author_sort Abdul Aziz, Nur Afiqah
title Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_short Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_full Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_fullStr Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_full_unstemmed Development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / Nur Afiqah Abdul Aziz
title_sort development of transgenic tobacco plants carrying the cinnamyl alcohol dehydrogenase 1 gene of oil palm / nur afiqah abdul aziz
publisher Faculty of Plantation and Agrotechnology
publishDate 2018
url http://ir.uitm.edu.my/id/eprint/22769/1/22769.pdf
http://ir.uitm.edu.my/id/eprint/22769/
_version_ 1685649648134389760
score 13.211869

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