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Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz

Phytochemical investigation on the bark of Malaysian Croton laevifolius Blume (Euphorbiaceae) was carried out with an intention of isolating and identifying its chemical constituents. The bark was successively extracted with the non polar (hexane), medium polar (dichloromethane) and polar (methanol)...

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Main Author: Aziz, Ahmad Nazif
Format: Book Section
Language:English
Published: Institute of Graduate Studies, UiTM 2018
Subjects:
Analytical chemistry
Online Access:http://ir.uitm.edu.my/id/eprint/22019/1/ABS_AHMAD%20NAZIF%20AZIZ%20TDRA%20VOL%2014%20IGS%2018.pdf
http://ir.uitm.edu.my/id/eprint/22019/
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spelling my.uitm.ir.220192018-11-12T07:14:13Z http://ir.uitm.edu.my/id/eprint/22019/ Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz Aziz, Ahmad Nazif Analytical chemistry Phytochemical investigation on the bark of Malaysian Croton laevifolius Blume (Euphorbiaceae) was carried out with an intention of isolating and identifying its chemical constituents. The bark was successively extracted with the non polar (hexane), medium polar (dichloromethane) and polar (methanol) organic solvents. These extracts were evaluated for cytotoxicity, anti-inflammatory and antidiabetic activities. The hexane extract showed potential cytotoxicity against MCF-7 cell line and mild cytotoxicity against A549, WRL-68, PC-3 and A375 cells while dichloromethane (DCM) extract indicated mild and selective activity against A549 and A375 only. The hexane and DCM extracts were subjected to isolation and purification using various chromatographic techniques such as Medium Pressure Liquid Chromatography (MPLC), Radial Chromatography (RC) and Recycling Preparative High Performance Liquid Chromatography (RHPLC). Structures of the chemical compounds were elucidated using various spectroscopic techniques such as UV, IR, 1D-NMR (1H, 13C, DEPT and APT), 2D-NMR (COSY, HSQC, HMBC and NOESY) and mass spectrometry. This study has led to the isolation of fourteen compounds, in which seven new and one known clerodane type diterpene named crovatin, as well as one eudesmane-type sesquiterpene named crytomeridiol were isolated from the DCM extract. This is the first occurrence of cryptomeridiol in Croton species. The new diterpenes were deduced as laevifin A, laevifin B, laevifin C, laevifin D, laevifin E, laevifin F and laevifin G. Subsequently, the hexane extract yielded laevifin B, three oleanane triterpenes: P-amyrin, (3-amyrone (MS) = Main Supervisor (CS) = Co Supervisor and acetyl aleuritolic acid; one steroid (p-sitostenone) and one flavonoid named pachypodol… Institute of Graduate Studies, UiTM 2018 Book Section PeerReviewed text en http://ir.uitm.edu.my/id/eprint/22019/1/ABS_AHMAD%20NAZIF%20AZIZ%20TDRA%20VOL%2014%20IGS%2018.pdf Aziz, Ahmad Nazif (2018) Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz. In: The Doctoral Research Abstracts. IGS Biannual Publication, 14 . Institute of Graduate Studies, UiTM, Shah Alam.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Analytical chemistry
spellingShingle Analytical chemistry
Aziz, Ahmad Nazif
Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz
description Phytochemical investigation on the bark of Malaysian Croton laevifolius Blume (Euphorbiaceae) was carried out with an intention of isolating and identifying its chemical constituents. The bark was successively extracted with the non polar (hexane), medium polar (dichloromethane) and polar (methanol) organic solvents. These extracts were evaluated for cytotoxicity, anti-inflammatory and antidiabetic activities. The hexane extract showed potential cytotoxicity against MCF-7 cell line and mild cytotoxicity against A549, WRL-68, PC-3 and A375 cells while dichloromethane (DCM) extract indicated mild and selective activity against A549 and A375 only. The hexane and DCM extracts were subjected to isolation and purification using various chromatographic techniques such as Medium Pressure Liquid Chromatography (MPLC), Radial Chromatography (RC) and Recycling Preparative High Performance Liquid Chromatography (RHPLC). Structures of the chemical compounds were elucidated using various spectroscopic techniques such as UV, IR, 1D-NMR (1H, 13C, DEPT and APT), 2D-NMR (COSY, HSQC, HMBC and NOESY) and mass spectrometry. This study has led to the isolation of fourteen compounds, in which seven new and one known clerodane type diterpene named crovatin, as well as one eudesmane-type sesquiterpene named crytomeridiol were isolated from the DCM extract. This is the first occurrence of cryptomeridiol in Croton species. The new diterpenes were deduced as laevifin A, laevifin B, laevifin C, laevifin D, laevifin E, laevifin F and laevifin G. Subsequently, the hexane extract yielded laevifin B, three oleanane triterpenes: P-amyrin, (3-amyrone (MS) = Main Supervisor (CS) = Co Supervisor and acetyl aleuritolic acid; one steroid (p-sitostenone) and one flavonoid named pachypodol…
format Book Section
author Aziz, Ahmad Nazif
author_facet Aziz, Ahmad Nazif
author_sort Aziz, Ahmad Nazif
title Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz
title_short Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz
title_full Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz
title_fullStr Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz
title_full_unstemmed Chemical constituents of Croton laevifolius Blume bark / Ahmad Nazif Aziz
title_sort chemical constituents of croton laevifolius blume bark / ahmad nazif aziz
publisher Institute of Graduate Studies, UiTM
publishDate 2018
url http://ir.uitm.edu.my/id/eprint/22019/1/ABS_AHMAD%20NAZIF%20AZIZ%20TDRA%20VOL%2014%20IGS%2018.pdf
http://ir.uitm.edu.my/id/eprint/22019/
_version_ 1685649544032813056
score 13.211869

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