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Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan

Cryopreservation of embryos is an essential procedure in all assisted conception units. Prediction of embryonic cryotolerance and developmental capacity after cryopreservation has previously relied on morphological assessment and observation on developmental capacity (Kuleshova et al., 2001; Han et...

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Main Authors: Noor Khan, Nor Ashikin Mohamed, Rajikin, Mohd. Hamim, Froemming, Gabriele Ruth Anisah, Abdul Satar, Nuraliza, Ibrahim, Mohd Johari, Dasiman, Razif
Format: Research Reports
Language:English
Published: Research Management Institute (RMI) 2012
Subjects:
Biology
Online Access:http://ir.uitm.edu.my/id/eprint/21025/1/LP_NOR%20ASHIKIN%20MOHAMED%20NOOR%20KHAN%20RMI%2012_5.pdf
http://ir.uitm.edu.my/id/eprint/21025/
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spelling my.uitm.ir.210252018-08-21T04:01:51Z http://ir.uitm.edu.my/id/eprint/21025/ Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan Noor Khan, Nor Ashikin Mohamed Rajikin, Mohd. Hamim Froemming, Gabriele Ruth Anisah Abdul Satar, Nuraliza Ibrahim, Mohd Johari Dasiman, Razif Biology Cryopreservation of embryos is an essential procedure in all assisted conception units. Prediction of embryonic cryotolerance and developmental capacity after cryopreservation has previously relied on morphological assessment and observation on developmental capacity (Kuleshova et al., 2001; Han et al., 2003). Technological advancements in recent years has allowed for high-resolution microscopy in which details of ultrastructure at the organelle level may be used to select viable embryos for transfer (Dailey et al., 2006; Yamagata et al., 2009). In these studies, disruption to cytoskeletal components and mitochondrial distribution are observed and used in the selection of viable embryos. Although selection done based on qualitative morphological criteria produces valuable information on the physical damages after cryopreservation, the use of quantitative methods has proven to be more accurate in predicting survivability (Stokes, et al., 2007). Metabolomics is a useful quantitative tool to predict viability of embryos for transfer (Katz-Jaffe et al., 2009). With regard to metabolomics, amino acid turnover in the culture media (Houghton et al. 2002: Brison et al. 2007) and measurements of key functional groups using near-infrared spectroscopy (NIR) (Vergouw et al. 2009) has been used effectively to predict embryo developmental capacity. Research Management Institute (RMI) 2012 Research Reports NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/21025/1/LP_NOR%20ASHIKIN%20MOHAMED%20NOOR%20KHAN%20RMI%2012_5.pdf Noor Khan, Nor Ashikin Mohamed and Rajikin, Mohd. Hamim and Froemming, Gabriele Ruth Anisah and Abdul Satar, Nuraliza and Ibrahim, Mohd Johari and Dasiman, Razif (2012) Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan. [Research Reports] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Biology
spellingShingle Biology
Noor Khan, Nor Ashikin Mohamed
Rajikin, Mohd. Hamim
Froemming, Gabriele Ruth Anisah
Abdul Satar, Nuraliza
Ibrahim, Mohd Johari
Dasiman, Razif
Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan
description Cryopreservation of embryos is an essential procedure in all assisted conception units. Prediction of embryonic cryotolerance and developmental capacity after cryopreservation has previously relied on morphological assessment and observation on developmental capacity (Kuleshova et al., 2001; Han et al., 2003). Technological advancements in recent years has allowed for high-resolution microscopy in which details of ultrastructure at the organelle level may be used to select viable embryos for transfer (Dailey et al., 2006; Yamagata et al., 2009). In these studies, disruption to cytoskeletal components and mitochondrial distribution are observed and used in the selection of viable embryos. Although selection done based on qualitative morphological criteria produces valuable information on the physical damages after cryopreservation, the use of quantitative methods has proven to be more accurate in predicting survivability (Stokes, et al., 2007). Metabolomics is a useful quantitative tool to predict viability of embryos for transfer (Katz-Jaffe et al., 2009). With regard to metabolomics, amino acid turnover in the culture media (Houghton et al. 2002: Brison et al. 2007) and measurements of key functional groups using near-infrared spectroscopy (NIR) (Vergouw et al. 2009) has been used effectively to predict embryo developmental capacity.
format Research Reports
author Noor Khan, Nor Ashikin Mohamed
Rajikin, Mohd. Hamim
Froemming, Gabriele Ruth Anisah
Abdul Satar, Nuraliza
Ibrahim, Mohd Johari
Dasiman, Razif
author_facet Noor Khan, Nor Ashikin Mohamed
Rajikin, Mohd. Hamim
Froemming, Gabriele Ruth Anisah
Abdul Satar, Nuraliza
Ibrahim, Mohd Johari
Dasiman, Razif
author_sort Noor Khan, Nor Ashikin Mohamed
title Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan
title_short Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan
title_full Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan
title_fullStr Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan
title_full_unstemmed Elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / Nor Ashikin Mohamed Noor Khan
title_sort elucidation of the identification mechanism of murine embryonic cryotolerance through metabolomic analyses and the study of cellular ultrastructures / nor ashikin mohamed noor khan
publisher Research Management Institute (RMI)
publishDate 2012
url http://ir.uitm.edu.my/id/eprint/21025/1/LP_NOR%20ASHIKIN%20MOHAMED%20NOOR%20KHAN%20RMI%2012_5.pdf
http://ir.uitm.edu.my/id/eprint/21025/
_version_ 1685649410515533824
score 13.211869

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