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Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan

In the current era of market globalization, people in the world could not evade from imported food products. The demand for imported food products such as chocolates, biscuits and sweets are projected to escalate steadily over the next decade as a result of increasing consumption. Unfortunately, mos...

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Main Author: Hassan, Khairunnisa
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/17906/2/TM_KHAIRUNNISA%20HASSAN%20AS%2016_5.pdf
https://ir.uitm.edu.my/id/eprint/17906/
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spelling my.uitm.ir.179062022-03-08T07:18:08Z https://ir.uitm.edu.my/id/eprint/17906/ Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan Hassan, Khairunnisa In the current era of market globalization, people in the world could not evade from imported food products. The demand for imported food products such as chocolates, biscuits and sweets are projected to escalate steadily over the next decade as a result of increasing consumption. Unfortunately, most of the imported food products do not have Halal Logo or with doubted Halal Logo. The demand for Halal food and other Islamic consumer goods is increasing. This study will be beneficial to provide new information of Halal products and easier for Muslim to choose the permissible products according to Syariah Law. The main objective of this study was to determine of suspected Non Halal processed food products by using porcine mitochondrial 12S rDNA and porcine leptin gene. A total of 66 samples of suspected Non Halal food products were screened for porcine mitochondrial 12S rDNA gene and porcine leptin gene primer pairs from the genomic DNA. The PCR products were separated on 2% agarose gel and visualized under UV light. Thirty seven samples were positive with mitochondrial 12S rDNA gene whilst 59 samples were positive with leptin gene. From these, 33 were positive with both primers. These results indicate that the samples of processed food products contained porcine derivatives. From the detection of the DNA products by using the two set of primers, leptin gene was concluded to be more specific than the mitochondrial 12S rDNA gene. Some of the PCR products of processed food products of mitochondrial 12S rDNA gene and leptin gene were sent to Genomic Bioscience & Technology Company for DNA sequencing. Then, the sequences of the DNA were used for sequence alignment in order to get a probe specific to Halal food. Two probes were obtained, one with 24 mers and 13 mers, respectively. Mitochondrial 12S rDNA gene was chosen to make a probe because it has more quality DNA for a probe compared to leptin gene. In addition, findings from this research also provide new information in the detection of pork in foods products for Halal authentication. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/17906/2/TM_KHAIRUNNISA%20HASSAN%20AS%2016_5.pdf ID17906 Hassan, Khairunnisa (2016) Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan. Masters thesis, thesis, Universiti Teknologi MARA.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description In the current era of market globalization, people in the world could not evade from imported food products. The demand for imported food products such as chocolates, biscuits and sweets are projected to escalate steadily over the next decade as a result of increasing consumption. Unfortunately, most of the imported food products do not have Halal Logo or with doubted Halal Logo. The demand for Halal food and other Islamic consumer goods is increasing. This study will be beneficial to provide new information of Halal products and easier for Muslim to choose the permissible products according to Syariah Law. The main objective of this study was to determine of suspected Non Halal processed food products by using porcine mitochondrial 12S rDNA and porcine leptin gene. A total of 66 samples of suspected Non Halal food products were screened for porcine mitochondrial 12S rDNA gene and porcine leptin gene primer pairs from the genomic DNA. The PCR products were separated on 2% agarose gel and visualized under UV light. Thirty seven samples were positive with mitochondrial 12S rDNA gene whilst 59 samples were positive with leptin gene. From these, 33 were positive with both primers. These results indicate that the samples of processed food products contained porcine derivatives. From the detection of the DNA products by using the two set of primers, leptin gene was concluded to be more specific than the mitochondrial 12S rDNA gene. Some of the PCR products of processed food products of mitochondrial 12S rDNA gene and leptin gene were sent to Genomic Bioscience & Technology Company for DNA sequencing. Then, the sequences of the DNA were used for sequence alignment in order to get a probe specific to Halal food. Two probes were obtained, one with 24 mers and 13 mers, respectively. Mitochondrial 12S rDNA gene was chosen to make a probe because it has more quality DNA for a probe compared to leptin gene. In addition, findings from this research also provide new information in the detection of pork in foods products for Halal authentication.
format Thesis
author Hassan, Khairunnisa
spellingShingle Hassan, Khairunnisa
Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
author_facet Hassan, Khairunnisa
author_sort Hassan, Khairunnisa
title Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_short Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_full Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_fullStr Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_full_unstemmed Determination of suspected non halal food products by using porcine mitochondrial 12s rDNA and porcine leptin gene / Khairunnisa Hassan
title_sort determination of suspected non halal food products by using porcine mitochondrial 12s rdna and porcine leptin gene / khairunnisa hassan
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/17906/2/TM_KHAIRUNNISA%20HASSAN%20AS%2016_5.pdf
https://ir.uitm.edu.my/id/eprint/17906/
_version_ 1726795633781637120
score 13.211869

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