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Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim

These present works have focus on the isolation of galactomannan and synthesis of galactomannan nanoparticles. Galactomannan nanoparticles possess attractive properties such as biodegradable and biocompatible; significant in biomedical applications. Galactomannan was isolated from the mature seeds o...

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Main Author: Rahim, Nurnadiah
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/17875/2/17875.pdf
https://ir.uitm.edu.my/id/eprint/17875/
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spelling my.uitm.ir.178752024-07-24T06:20:11Z https://ir.uitm.edu.my/id/eprint/17875/ Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim Rahim, Nurnadiah These present works have focus on the isolation of galactomannan and synthesis of galactomannan nanoparticles. Galactomannan nanoparticles possess attractive properties such as biodegradable and biocompatible; significant in biomedical applications. Galactomannan was isolated from the mature seeds of a local plant, petai belalang known scientifically as Leucaena leucocephala via water extraction followed by precipitation with ethanol and purified by ion-exchange chromatography. It was chemically analyzed by UV, NMR, FTIR, GPC and HPLC. Pure galactomannan was successfully isolated with a yield of 50.31 % (w/w). It consisted of D-mannose and Dgalactose with mannose: galactose (M/G) ratio of 1.01. The molecular weight of the galactomannan was found to be 64 kDa. Galactomannan obtained from this present work is soluble in water. Galactomannan were then modified to acrylated galactomannan (AcGA) which act as a precursor molecule for the preparation of crosslinked galactomannan nanoparticles (CL-GA). AcGA was synthesized using acryloyl chloride (AC) as a chemical modifier through esterification process. Trimethylolpropane triacrylate (TMPTA) was used as the crosslinker for the preparation of CL-GA. Successful conjugation of GA with acrylates was verified by ¹H NMR and FTIR spectroscopies. Thermal properties of pure galactomannan, AcGA and CL-GA were measured using DSC to complement spectroscopic results. TEM images revealed the particle size of AcGA to be in the range of 30-100 nm; and increased to 200-850 nm range after the crosslinking process. Efficiency of CL-GA to trap crystal violet dye as a model drug was followed by UV Vis Spectroscopy. Results showed that CL-GA is capable of trapping crystal violet dye and has the potential to be used as a drug carrier. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/17875/2/17875.pdf Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim. (2016) Masters thesis, thesis, Universiti Teknologi MARA.
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description These present works have focus on the isolation of galactomannan and synthesis of galactomannan nanoparticles. Galactomannan nanoparticles possess attractive properties such as biodegradable and biocompatible; significant in biomedical applications. Galactomannan was isolated from the mature seeds of a local plant, petai belalang known scientifically as Leucaena leucocephala via water extraction followed by precipitation with ethanol and purified by ion-exchange chromatography. It was chemically analyzed by UV, NMR, FTIR, GPC and HPLC. Pure galactomannan was successfully isolated with a yield of 50.31 % (w/w). It consisted of D-mannose and Dgalactose with mannose: galactose (M/G) ratio of 1.01. The molecular weight of the galactomannan was found to be 64 kDa. Galactomannan obtained from this present work is soluble in water. Galactomannan were then modified to acrylated galactomannan (AcGA) which act as a precursor molecule for the preparation of crosslinked galactomannan nanoparticles (CL-GA). AcGA was synthesized using acryloyl chloride (AC) as a chemical modifier through esterification process. Trimethylolpropane triacrylate (TMPTA) was used as the crosslinker for the preparation of CL-GA. Successful conjugation of GA with acrylates was verified by ¹H NMR and FTIR spectroscopies. Thermal properties of pure galactomannan, AcGA and CL-GA were measured using DSC to complement spectroscopic results. TEM images revealed the particle size of AcGA to be in the range of 30-100 nm; and increased to 200-850 nm range after the crosslinking process. Efficiency of CL-GA to trap crystal violet dye as a model drug was followed by UV Vis Spectroscopy. Results showed that CL-GA is capable of trapping crystal violet dye and has the potential to be used as a drug carrier.
format Thesis
author Rahim, Nurnadiah
spellingShingle Rahim, Nurnadiah
Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
author_facet Rahim, Nurnadiah
author_sort Rahim, Nurnadiah
title Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_short Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_full Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_fullStr Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_full_unstemmed Isolation and purification of galactomannan from Leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / Nurnadiah Rahim
title_sort isolation and purification of galactomannan from leucaena leucocephala and synthesis of crosslinked galactomannan nanoparticles / nurnadiah rahim
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/17875/2/17875.pdf
https://ir.uitm.edu.my/id/eprint/17875/
_version_ 1806422026445914112
score 13.211869

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