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In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail

In vitro regeneration of Ananas comosus L. Merr. 'MD2' (pineapple) was carried out using MD2 pineapple sucker as explant. In vitro regeneration was done by manipulating the combination of plant growth regulator between Indole Acetic Acid (IAA) and Kinetin (Kn). Eleven different combination...

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Main Author: Ismail, Norma Ziati
Format: Student Project
Language:English
Published: Faculty of Plantation and Agrotechnology 2015
Online Access:http://ir.uitm.edu.my/id/eprint/17078/1/PPb_NORMA%20ZIATI%20ISMAIL%20AT%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/17078/
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spelling my.uitm.ir.170782019-07-22T03:35:15Z http://ir.uitm.edu.my/id/eprint/17078/ In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail Ismail, Norma Ziati In vitro regeneration of Ananas comosus L. Merr. 'MD2' (pineapple) was carried out using MD2 pineapple sucker as explant. In vitro regeneration was done by manipulating the combination of plant growth regulator between Indole Acetic Acid (IAA) and Kinetin (Kn). Eleven different combination of IAA and Kn hormone was applied in MS medium with vitamins supplemented with 8.0g/L agar and 30g/L sucrose at combination of different concentration of IAA (0.00,0.05,0.10,0.15,0.20,0.25,0.30,0.35,0.40,0.45 and 0.5 mg/L) and Kn (0.0,0.50,1.00,1.50,2.00,2.50,3.00,3.50,4.00,4.50 and 5.00 mg/L) respectively and maintain at 25°C under 16 hours of photoperiod. The result shows that the sucker explant able to induce shoot proliferation on different combination of IAA and Kn. Optimum regeneration for the best result was obtained from MS medium supplemented with 0.35mg/L IAA and 3.50mg/L Kn. Shoot proliferation start undergo morphogenesis after 2 to 3 weeks culture on MS medium. In addition, this combination of plant growth regulator regenerated highest mean for the number of shoot regenerate per explant (2.98unit), the length of shoot (0.82cm), height of plantlet (1.01cm) and the weight of plantlet (1.42g) compared to other combination of IAA and Kn tested. However, all the explant showing different respond towards treatment given. Faculty of Plantation and Agrotechnology 2015 Student Project NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/17078/1/PPb_NORMA%20ZIATI%20ISMAIL%20AT%2015_5.pdf Ismail, Norma Ziati (2015) In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail. [Student Project] (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description In vitro regeneration of Ananas comosus L. Merr. 'MD2' (pineapple) was carried out using MD2 pineapple sucker as explant. In vitro regeneration was done by manipulating the combination of plant growth regulator between Indole Acetic Acid (IAA) and Kinetin (Kn). Eleven different combination of IAA and Kn hormone was applied in MS medium with vitamins supplemented with 8.0g/L agar and 30g/L sucrose at combination of different concentration of IAA (0.00,0.05,0.10,0.15,0.20,0.25,0.30,0.35,0.40,0.45 and 0.5 mg/L) and Kn (0.0,0.50,1.00,1.50,2.00,2.50,3.00,3.50,4.00,4.50 and 5.00 mg/L) respectively and maintain at 25°C under 16 hours of photoperiod. The result shows that the sucker explant able to induce shoot proliferation on different combination of IAA and Kn. Optimum regeneration for the best result was obtained from MS medium supplemented with 0.35mg/L IAA and 3.50mg/L Kn. Shoot proliferation start undergo morphogenesis after 2 to 3 weeks culture on MS medium. In addition, this combination of plant growth regulator regenerated highest mean for the number of shoot regenerate per explant (2.98unit), the length of shoot (0.82cm), height of plantlet (1.01cm) and the weight of plantlet (1.42g) compared to other combination of IAA and Kn tested. However, all the explant showing different respond towards treatment given.
format Student Project
author Ismail, Norma Ziati
spellingShingle Ismail, Norma Ziati
In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail
author_facet Ismail, Norma Ziati
author_sort Ismail, Norma Ziati
title In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail
title_short In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail
title_full In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail
title_fullStr In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail
title_full_unstemmed In vitro regeneration of pineapple (Ananas comosus) l. Merr 'MD2' using different concentration of Indole Acetic Acid (IAA) and Kinetin (KN) hormone / Norma Ziati Ismail
title_sort in vitro regeneration of pineapple (ananas comosus) l. merr 'md2' using different concentration of indole acetic acid (iaa) and kinetin (kn) hormone / norma ziati ismail
publisher Faculty of Plantation and Agrotechnology
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/17078/1/PPb_NORMA%20ZIATI%20ISMAIL%20AT%2015_5.pdf
http://ir.uitm.edu.my/id/eprint/17078/
_version_ 1685648892854534144
score 13.211869

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