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The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin

Excessive amount of glucocorticoid [cortisol in human or corticosterone (CORT) in rodent] induces oxidative stress (OS) in the cell leading to DNA damage and it has been proven by previous studies. Conversely, it is well documented that tocotrienol (TCT), a potent antioxidant was able to protect ce...

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Main Author: Zainal Abidin, Shahidee
Format: Thesis
Language:English
Published: 2014
Subjects:
Embryology
Physiology of the embryo
Online Access:https://ir.uitm.edu.my/id/eprint/14344/1/14344.pdf
https://ir.uitm.edu.my/id/eprint/14344/
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spelling my.uitm.ir.143442022-09-05T04:10:13Z https://ir.uitm.edu.my/id/eprint/14344/ The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin Zainal Abidin, Shahidee Embryology Physiology of the embryo Excessive amount of glucocorticoid [cortisol in human or corticosterone (CORT) in rodent] induces oxidative stress (OS) in the cell leading to DNA damage and it has been proven by previous studies. Conversely, it is well documented that tocotrienol (TCT), a potent antioxidant was able to protect cells by neutralizing excessive reactive oxygen species (ROS). Hence, this study was designed to determine the effect of TCT supplementation on the quality and development of embryos and DNA damage level in embryos ofCORT-treated mice. Female mice were given TCT orally at three different doses i.e. 30, 60, and 90 mg kg" BW, concurrent with 10 mg kg" BW of CORT intraperitoneally (ip) for 14 days. Mice were superovulated and paired individually overnight with stud male mice. After 48 hours post-coitum, female mice were euthanized to collect 2-cell stage of embryos. The morphological observation and in vitro development of embryos were accessed and monitored under an inverted microscope and the percentage ofDNA damage was analysed via Comet assay. It was found that oral supplementation of 90 mg kg" BW of TCT in CORT-treated mice were able to normalize the number of fragmented embryos and improve the number of embryos that reach the blastocyst stage. No DNA damage was noted in all CORTtreated groups supplemented with TCT. Supplementation of TCT also suppresses the level of 8-hydroxy-2'-deoxyguanosine (8-0HdG) and restored catalase (CAT) activity toward control. The findings of this study indicate that TCT supplementation in CORT-treated mice was able to reverse the effect of CORT-induced fragmentation and oxidative DNA damage in embryos. Thus, the molecular mechanisms by which TCT suppresses oxidative stress and promotes the quality of embryo need to be investigated in detail in future studies 2014 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/14344/1/14344.pdf The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin. (2014) Masters thesis, thesis, Universiti Teknologi MARA. <http://terminalib.uitm.edu.my/14344.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Embryology
Physiology of the embryo
spellingShingle Embryology
Physiology of the embryo
Zainal Abidin, Shahidee
The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin
description Excessive amount of glucocorticoid [cortisol in human or corticosterone (CORT) in rodent] induces oxidative stress (OS) in the cell leading to DNA damage and it has been proven by previous studies. Conversely, it is well documented that tocotrienol (TCT), a potent antioxidant was able to protect cells by neutralizing excessive reactive oxygen species (ROS). Hence, this study was designed to determine the effect of TCT supplementation on the quality and development of embryos and DNA damage level in embryos ofCORT-treated mice. Female mice were given TCT orally at three different doses i.e. 30, 60, and 90 mg kg" BW, concurrent with 10 mg kg" BW of CORT intraperitoneally (ip) for 14 days. Mice were superovulated and paired individually overnight with stud male mice. After 48 hours post-coitum, female mice were euthanized to collect 2-cell stage of embryos. The morphological observation and in vitro development of embryos were accessed and monitored under an inverted microscope and the percentage ofDNA damage was analysed via Comet assay. It was found that oral supplementation of 90 mg kg" BW of TCT in CORT-treated mice were able to normalize the number of fragmented embryos and improve the number of embryos that reach the blastocyst stage. No DNA damage was noted in all CORTtreated groups supplemented with TCT. Supplementation of TCT also suppresses the level of 8-hydroxy-2'-deoxyguanosine (8-0HdG) and restored catalase (CAT) activity toward control. The findings of this study indicate that TCT supplementation in CORT-treated mice was able to reverse the effect of CORT-induced fragmentation and oxidative DNA damage in embryos. Thus, the molecular mechanisms by which TCT suppresses oxidative stress and promotes the quality of embryo need to be investigated in detail in future studies
format Thesis
author Zainal Abidin, Shahidee
author_facet Zainal Abidin, Shahidee
author_sort Zainal Abidin, Shahidee
title The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin
title_short The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin
title_full The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin
title_fullStr The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin
title_full_unstemmed The protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / Shahidee Zainal Abidin
title_sort protective role of tocotrienol on corticosterone induced oxidative stress during preimplantation embryonic development in mice / shahidee zainal abidin
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/14344/1/14344.pdf
https://ir.uitm.edu.my/id/eprint/14344/
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score 13.211869

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