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The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim

Marine macroalgae are important ecologically and commercially sources in many regions of the world especially Asian countries. The marine macroalgae especially seaweeds have been one of the richest and most promising sources of bioactive metabolites in pharmaceutical application. Due to the toxicity...

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Main Author: Zainal Abdim, Nur Fatihah
Format: Thesis
Language:English
Published: 2014
Subjects:
Cancer
Online Access:https://ir.uitm.edu.my/id/eprint/111676/1/111676.PDF
https://ir.uitm.edu.my/id/eprint/111676/
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spelling my.uitm.ir.1116762025-03-22T00:21:43Z https://ir.uitm.edu.my/id/eprint/111676/ The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim Zainal Abdim, Nur Fatihah Cancer Marine macroalgae are important ecologically and commercially sources in many regions of the world especially Asian countries. The marine macroalgae especially seaweeds have been one of the richest and most promising sources of bioactive metabolites in pharmaceutical application. Due to the toxicity of existing chemotherapeutic drugs, there is an urge to find an alternative source of chemotherapeutic agents. In this study, three marine endophytic fungi isolated from selected seaweeds (Gracilaria arcuata Zanardini, Gracilaria coronapifolia J.Agard and Padina minor Yamada) were investigated. The marine endophytic fungi isolated from these selected seaweeds were grown on two types of media which PDA supplemented with 1 % and 3% artificial sea salt. The cytotoxicity of the marine endophytic fungi (MY, CN and PA7) was evaluated by using MTT assay on liver cancer cell line (HepG2). The lowest IC50 values were observed at 24 hours of incubation time. Three out of six extracts showed very active cytotoxic activity MY 3%; 0.10±0.00µgml⁻¹, CN 3%; 2.45±1.77µgmr1, CN 1 %; 4.00±2.83µgml⁻¹ and another three showed active cytotoxic activity MY 1%; 10.50±0.71µgml⁻¹, PA7 1%; 12.00±2.83µgml⁻¹, PA7 3%; 11.00±1.41µgml⁻¹. MV 3% (10.50±0.71µgml⁻¹) exhibited the most pronounced extract. Among the extracts, MV 3% showed significant difference with MV 1 %, PA1 1 % and PA1 3% (p<0.05). In facts, among six tested extracts, the IC50 obtained for MV 3% extract was lower as compared to the IC50 of 5-FU at different incubation time (48 hours; 0.38 µgml⁻¹, 72 hours; 0.25 µgml⁻¹ and 96 hours; 0.38µgml⁻¹). Among the extracts, significance difference were only observed between MV 1 % and MV 3% (p<0.05) extracts. In this present study, 1 % of artificial sea salt was sufficient to exhibit cytotoxic effect except for marine endophytic fungi extract (MV 3%). Therefore, these tested marine endophytic fungi extracts might have potential as an alternative source against HepG2 cells and worth to be further studied as anticancer agents. 2014 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/111676/1/111676.PDF The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim. (2014) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
spellingShingle Cancer
Zainal Abdim, Nur Fatihah
The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim
description Marine macroalgae are important ecologically and commercially sources in many regions of the world especially Asian countries. The marine macroalgae especially seaweeds have been one of the richest and most promising sources of bioactive metabolites in pharmaceutical application. Due to the toxicity of existing chemotherapeutic drugs, there is an urge to find an alternative source of chemotherapeutic agents. In this study, three marine endophytic fungi isolated from selected seaweeds (Gracilaria arcuata Zanardini, Gracilaria coronapifolia J.Agard and Padina minor Yamada) were investigated. The marine endophytic fungi isolated from these selected seaweeds were grown on two types of media which PDA supplemented with 1 % and 3% artificial sea salt. The cytotoxicity of the marine endophytic fungi (MY, CN and PA7) was evaluated by using MTT assay on liver cancer cell line (HepG2). The lowest IC50 values were observed at 24 hours of incubation time. Three out of six extracts showed very active cytotoxic activity MY 3%; 0.10±0.00µgml⁻¹, CN 3%; 2.45±1.77µgmr1, CN 1 %; 4.00±2.83µgml⁻¹ and another three showed active cytotoxic activity MY 1%; 10.50±0.71µgml⁻¹, PA7 1%; 12.00±2.83µgml⁻¹, PA7 3%; 11.00±1.41µgml⁻¹. MV 3% (10.50±0.71µgml⁻¹) exhibited the most pronounced extract. Among the extracts, MV 3% showed significant difference with MV 1 %, PA1 1 % and PA1 3% (p<0.05). In facts, among six tested extracts, the IC50 obtained for MV 3% extract was lower as compared to the IC50 of 5-FU at different incubation time (48 hours; 0.38 µgml⁻¹, 72 hours; 0.25 µgml⁻¹ and 96 hours; 0.38µgml⁻¹). Among the extracts, significance difference were only observed between MV 1 % and MV 3% (p<0.05) extracts. In this present study, 1 % of artificial sea salt was sufficient to exhibit cytotoxic effect except for marine endophytic fungi extract (MV 3%). Therefore, these tested marine endophytic fungi extracts might have potential as an alternative source against HepG2 cells and worth to be further studied as anticancer agents.
format Thesis
author Zainal Abdim, Nur Fatihah
author_facet Zainal Abdim, Nur Fatihah
author_sort Zainal Abdim, Nur Fatihah
title The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim
title_short The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim
title_full The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim
title_fullStr The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim
title_full_unstemmed The cytotoxic effect of red and brown Malaysian sea weeds against liver cancer cell line (HepG2) / Nur Fatihah Zainal Abdim
title_sort cytotoxic effect of red and brown malaysian sea weeds against liver cancer cell line (hepg2) / nur fatihah zainal abdim
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/111676/1/111676.PDF
https://ir.uitm.edu.my/id/eprint/111676/
_version_ 1827446676045955072
score 13.250246

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