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Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab

Honeys have been proven to have an anti-oxidant and antitumor properties and Gelam honey was confirmed to have an ability of suppressing colon cancer cell growth. However, there are not many scientific studies that have been conducted to investigate the antitumor effects of Malaysian Gelam honey on...

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Main Author: A. Wahab, Siti Norfazila
Format: Thesis
Language:English
Published: 2014
Subjects:
Cancer
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/111555/1/111555.PDF
https://ir.uitm.edu.my/id/eprint/111555/
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spelling my.uitm.ir.1115552025-03-23T18:17:49Z https://ir.uitm.edu.my/id/eprint/111555/ Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab A. Wahab, Siti Norfazila Cancer Pharmacopoeias Honeys have been proven to have an anti-oxidant and antitumor properties and Gelam honey was confirmed to have an ability of suppressing colon cancer cell growth. However, there are not many scientific studies that have been conducted to investigate the antitumor effects of Malaysian Gelam honey on lung cancer cells. ALPP gene code for alkaline phosphatase, which function in the regulation of phosphorylation, where phosphorylation play important and significant role in regulating the function of tumor suppressor protein, p53.The study is an initial attempt to investigate the relative expression of ALPP gene in lung cancer cell upon treatment with Gelam honey by using real time reverse transcription polymerase chain reaction. For the gene expression, the total RNA which is extracted from the untreated and treated lung cancer cell was reverse transcripted into cDNA and amplified with qRTPCR. The relative quantification of gene expression is calculated using Delta-delta Ct method. The result of this study revealed that the expression ALPP gene in treated lung cancer cell with Gelam honey is up­ regulated. From the normalization of gene expression using 2-△△Ct method, the ALPP gene is increased by 14.42 folds in the lung cancer cell which was treated with Gelam honey. Hence it can be concluded that ALPP gene showed different expression when treated with Gelam honey, where it is up-regulated. This up-regulation of ALPP gene showed that Gelam honey possessed therapeutic effect on lung cancer as up-regulation of ALPP gene will inhibit the growth of lung cancer cell by activated the p53 tumor suppressor protein. 2014 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/111555/1/111555.PDF Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab. (2014) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
Pharmacopoeias
spellingShingle Cancer
Pharmacopoeias
A. Wahab, Siti Norfazila
Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab
description Honeys have been proven to have an anti-oxidant and antitumor properties and Gelam honey was confirmed to have an ability of suppressing colon cancer cell growth. However, there are not many scientific studies that have been conducted to investigate the antitumor effects of Malaysian Gelam honey on lung cancer cells. ALPP gene code for alkaline phosphatase, which function in the regulation of phosphorylation, where phosphorylation play important and significant role in regulating the function of tumor suppressor protein, p53.The study is an initial attempt to investigate the relative expression of ALPP gene in lung cancer cell upon treatment with Gelam honey by using real time reverse transcription polymerase chain reaction. For the gene expression, the total RNA which is extracted from the untreated and treated lung cancer cell was reverse transcripted into cDNA and amplified with qRTPCR. The relative quantification of gene expression is calculated using Delta-delta Ct method. The result of this study revealed that the expression ALPP gene in treated lung cancer cell with Gelam honey is up­ regulated. From the normalization of gene expression using 2-△△Ct method, the ALPP gene is increased by 14.42 folds in the lung cancer cell which was treated with Gelam honey. Hence it can be concluded that ALPP gene showed different expression when treated with Gelam honey, where it is up-regulated. This up-regulation of ALPP gene showed that Gelam honey possessed therapeutic effect on lung cancer as up-regulation of ALPP gene will inhibit the growth of lung cancer cell by activated the p53 tumor suppressor protein.
format Thesis
author A. Wahab, Siti Norfazila
author_facet A. Wahab, Siti Norfazila
author_sort A. Wahab, Siti Norfazila
title Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab
title_short Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab
title_full Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab
title_fullStr Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab
title_full_unstemmed Relative expression of ALPP gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / Siti Norfazila A. Wahab
title_sort relative expression of alpp gene in lung cancer induced by gelam honey by quantitative reverse transcription polymerase chain reaction / siti norfazila a. wahab
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/111555/1/111555.PDF
https://ir.uitm.edu.my/id/eprint/111555/
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score 13.250246

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