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The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil

In recent years, generally about 30,000 new compounds from marine natural products have been explored and identified. This number of findings reflected the great potential of marine natural products as a source of novel chemicals classes. Due to the efficacy limitation of the available anticancer dr...

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書誌詳細
第一著者: Mohd Jalil, Syuhada
フォーマット: 学位論文
言語:English
出版事項: 2014
主題:
Cancer
オンライン･アクセス:https://ir.uitm.edu.my/id/eprint/111120/1/111120.PDF
https://ir.uitm.edu.my/id/eprint/111120/
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spelling my.uitm.ir.1111202025-03-04T23:14:28Z https://ir.uitm.edu.my/id/eprint/111120/ The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil Mohd Jalil, Syuhada Cancer In recent years, generally about 30,000 new compounds from marine natural products have been explored and identified. This number of findings reflected the great potential of marine natural products as a source of novel chemicals classes. Due to the efficacy limitation of the available anticancer drugs in liver cancer treatment, there is an urge to seek new candidate for alternatives drugs. In search for new source of active metabolites, marine endophytic fungi isolated from seaweeds were chosen to be further studied. Hence, in this study, three marine endophytic fungi (ED 1, ED2 and UF) isolated from red (Acanthophora spicifera (M. Vahl) Borgesen) and green Malaysian seaweeds ( Chaetomorpha minima F. S Collins & Hervey 1917) were grown on two types of media, PDA with 1 % and 3% of artificial sea salt (PDA 1 % and PDA 3%). Marine endophytic fungi extracts were investigated for their cytotoxic effect against HepG2 cells by using MIT assay. In this presence study, the lowest IC50 values were observed at 72 hours incubation times. Extracts with the IC5o values less than 50µg/ml [UF 1 % (16.5± l.5µg/ml), EDI 1 % (36 ± 16.00µg/ml) and UF 3% (42.5± 12.5µg/ml)] were considered to have promising cytotoxic activity. Only one extract [UF 1 % % (16.5± l.5µg/ml)] showed active cytotoxic activity whereas two extracts [(EDI 1 % (36 ± 16.00µg/ml) and UF 3% (42.5± 12.5µg/ml)] had moderate cytotoxic activity. As for salinity, endophytic fungi media supplemented with 1 % artificial sea salt was sufficient in providing cytotoxic effect against HepG2 cells as compared to 3%. Therefore, the cytotoxic effects exhibited by these three extracts against HepG2 cells could be considered as suitable candidates for further studies as anticancer agents. 2014 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/111120/1/111120.PDF The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil. (2014) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
spellingShingle Cancer
Mohd Jalil, Syuhada
The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil
description In recent years, generally about 30,000 new compounds from marine natural products have been explored and identified. This number of findings reflected the great potential of marine natural products as a source of novel chemicals classes. Due to the efficacy limitation of the available anticancer drugs in liver cancer treatment, there is an urge to seek new candidate for alternatives drugs. In search for new source of active metabolites, marine endophytic fungi isolated from seaweeds were chosen to be further studied. Hence, in this study, three marine endophytic fungi (ED 1, ED2 and UF) isolated from red (Acanthophora spicifera (M. Vahl) Borgesen) and green Malaysian seaweeds ( Chaetomorpha minima F. S Collins & Hervey 1917) were grown on two types of media, PDA with 1 % and 3% of artificial sea salt (PDA 1 % and PDA 3%). Marine endophytic fungi extracts were investigated for their cytotoxic effect against HepG2 cells by using MIT assay. In this presence study, the lowest IC50 values were observed at 72 hours incubation times. Extracts with the IC5o values less than 50µg/ml [UF 1 % (16.5± l.5µg/ml), EDI 1 % (36 ± 16.00µg/ml) and UF 3% (42.5± 12.5µg/ml)] were considered to have promising cytotoxic activity. Only one extract [UF 1 % % (16.5± l.5µg/ml)] showed active cytotoxic activity whereas two extracts [(EDI 1 % (36 ± 16.00µg/ml) and UF 3% (42.5± 12.5µg/ml)] had moderate cytotoxic activity. As for salinity, endophytic fungi media supplemented with 1 % artificial sea salt was sufficient in providing cytotoxic effect against HepG2 cells as compared to 3%. Therefore, the cytotoxic effects exhibited by these three extracts against HepG2 cells could be considered as suitable candidates for further studies as anticancer agents.
format Thesis
author Mohd Jalil, Syuhada
author_facet Mohd Jalil, Syuhada
author_sort Mohd Jalil, Syuhada
title The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil
title_short The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil
title_full The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil
title_fullStr The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil
title_full_unstemmed The cytotoxic effect of red and green Malaysian sea weeds against liver cancer cell lines (HepG2) / Syuhada Mohd Jalil
title_sort cytotoxic effect of red and green malaysian sea weeds against liver cancer cell lines (hepg2) / syuhada mohd jalil
publishDate 2014
url https://ir.uitm.edu.my/id/eprint/111120/1/111120.PDF
https://ir.uitm.edu.my/id/eprint/111120/
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score 13.250246

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