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Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh

Microbial transformation usage was very crucial long time ago. The scientists found that this method have more advantages compare to chemical synthesis method. The criteria of microbial itself such as regio- and stereoselective allow compound modification to occur and time consuming for the reaction...

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Main Author: Mat Jusoh, Siti Noor Aisyah
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Pharmaceutical chemistry
Online Access:https://ir.uitm.edu.my/id/eprint/110524/1/110524.PDF
https://ir.uitm.edu.my/id/eprint/110524/
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spelling my.uitm.ir.1105242025-02-21T23:43:34Z https://ir.uitm.edu.my/id/eprint/110524/ Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh Mat Jusoh, Siti Noor Aisyah Pharmacopoeias Pharmaceutical chemistry Microbial transformation usage was very crucial long time ago. The scientists found that this method have more advantages compare to chemical synthesis method. The criteria of microbial itself such as regio- and stereoselective allow compound modification to occur and time consuming for the reaction can be less. By having specific microbes, it can help transformation of the compound of interest at specific time. In this study, bryonolic acid is UV inactive compound has been used as substrate and undergone fermentation with several endophytes. The extraction of all samples were analyzed using TLC and HPLC. The suitable ratio for TLC was 60% hexane and 40% ethyl acetate while for HPLC that equipped with ELSD detector was used since bryonolic acid is UV inactive. Only three samples were selected to do HPLC profiling in which Pseudomonas aeruginosa, Staphylococcus aureus and Trichothecium roseum. The HPLC profiles were compared and Pseudomonas aeruginosa showed significant result in both TLC and HPLC. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/110524/1/110524.PDF Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
Pharmaceutical chemistry
spellingShingle Pharmacopoeias
Pharmaceutical chemistry
Mat Jusoh, Siti Noor Aisyah
Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh
description Microbial transformation usage was very crucial long time ago. The scientists found that this method have more advantages compare to chemical synthesis method. The criteria of microbial itself such as regio- and stereoselective allow compound modification to occur and time consuming for the reaction can be less. By having specific microbes, it can help transformation of the compound of interest at specific time. In this study, bryonolic acid is UV inactive compound has been used as substrate and undergone fermentation with several endophytes. The extraction of all samples were analyzed using TLC and HPLC. The suitable ratio for TLC was 60% hexane and 40% ethyl acetate while for HPLC that equipped with ELSD detector was used since bryonolic acid is UV inactive. Only three samples were selected to do HPLC profiling in which Pseudomonas aeruginosa, Staphylococcus aureus and Trichothecium roseum. The HPLC profiles were compared and Pseudomonas aeruginosa showed significant result in both TLC and HPLC.
format Thesis
author Mat Jusoh, Siti Noor Aisyah
author_facet Mat Jusoh, Siti Noor Aisyah
author_sort Mat Jusoh, Siti Noor Aisyah
title Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh
title_short Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh
title_full Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh
title_fullStr Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh
title_full_unstemmed Microbial biotransformation of bryonolic acid / Siti Noor Aisyah Mat Jusoh
title_sort microbial biotransformation of bryonolic acid / siti noor aisyah mat jusoh
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/110524/1/110524.PDF
https://ir.uitm.edu.my/id/eprint/110524/
_version_ 1825165198425587712
score 13.239859

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