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Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian

Amniotic fluid (AF) is a promising tool for future drug discovery and regenerative medicine due to the presence of amniotic fluid stem cells (AFSCs). Previous study has reported the presence of broadly multipotent stem cells from full term AF which corporate safer harvesting approach compared to the...

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Main Author: Damian, Danie Dean
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:https://ir.uitm.edu.my/id/eprint/110424/1/110424.PDF
https://ir.uitm.edu.my/id/eprint/110424/
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spelling my.uitm.ir.1104242025-02-21T23:55:03Z https://ir.uitm.edu.my/id/eprint/110424/ Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian Damian, Danie Dean Pharmacopoeias Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Amniotic fluid (AF) is a promising tool for future drug discovery and regenerative medicine due to the presence of amniotic fluid stem cells (AFSCs). Previous study has reported the presence of broadly multipotent stem cells from full term AF which corporate safer harvesting approach compared to the AF harvested from the mid-term pregnancy. However, the profiles of stem cells from full term pregnancy are not fully characterized. Therefore, this research was conducted to characterize and determine whether the AFS cells from full term pregnancy possesses similar characteristics as the AFS cells of second term pregnancy through the expression of adult stem cell markers CD34, CD45 and CD105 using flow cytometry. AF cells were successfully expanded, subcultured and harvested prior to cell staining. The cells were stained with CD34, CD45, and CD105 which then analysed with flow cytometry. Based on the result obtained, the cells has low expression of CD105 (7.42%) and no expression of CD34 and CD45. Therefore, the cell possesses more mesenchymal characteristics than hematopoietic characteristics. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/110424/1/110424.PDF Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle Pharmacopoeias
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Damian, Danie Dean
Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian
description Amniotic fluid (AF) is a promising tool for future drug discovery and regenerative medicine due to the presence of amniotic fluid stem cells (AFSCs). Previous study has reported the presence of broadly multipotent stem cells from full term AF which corporate safer harvesting approach compared to the AF harvested from the mid-term pregnancy. However, the profiles of stem cells from full term pregnancy are not fully characterized. Therefore, this research was conducted to characterize and determine whether the AFS cells from full term pregnancy possesses similar characteristics as the AFS cells of second term pregnancy through the expression of adult stem cell markers CD34, CD45 and CD105 using flow cytometry. AF cells were successfully expanded, subcultured and harvested prior to cell staining. The cells were stained with CD34, CD45, and CD105 which then analysed with flow cytometry. Based on the result obtained, the cells has low expression of CD105 (7.42%) and no expression of CD34 and CD45. Therefore, the cell possesses more mesenchymal characteristics than hematopoietic characteristics.
format Thesis
author Damian, Danie Dean
author_facet Damian, Danie Dean
author_sort Damian, Danie Dean
title Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian
title_short Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian
title_full Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian
title_fullStr Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian
title_full_unstemmed Expression of adult stem cell markers in full-term rat C-kit positive amniotic fluid cells / Danie Dean Damian
title_sort expression of adult stem cell markers in full-term rat c-kit positive amniotic fluid cells / danie dean damian
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/110424/1/110424.PDF
https://ir.uitm.edu.my/id/eprint/110424/
_version_ 1825165187997499392
score 13.239859

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