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Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni

The in vitro study on immunology status is performed to determine the immunomodulatory effect of extracts on cell lines. The purpose of this study is to investigate the immunomodulatory response of Myrmecodia platytyrea hexane and methanol extracts toward Wistar rat’s lymphocytes and to study the cy...

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Main Author: Jafni, Khadijah
Format: Student Project
Language:English
Published: 2012
Subjects:
Immunology
Online Access:https://ir.uitm.edu.my/id/eprint/110010/1/110010.pdf
https://ir.uitm.edu.my/id/eprint/110010/
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spelling my.uitm.ir.1100102025-02-07T05:04:40Z https://ir.uitm.edu.my/id/eprint/110010/ Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni Jafni, Khadijah Immunology The in vitro study on immunology status is performed to determine the immunomodulatory effect of extracts on cell lines. The purpose of this study is to investigate the immunomodulatory response of Myrmecodia platytyrea hexane and methanol extracts toward Wistar rat’s lymphocytes and to study the cytotoxic effect of both extracts on macrophage RAW 264.7. Both experiments were using MTS assay to get rate of proliferation and rate of cell death. In determining the lymphocyte proliferation activity, mitogen such as PHA was used as a positive control. The concentrations of Myrmecodia platytyrea of both extracts used in this experiment are 0.0001, 0.001, 0.01, 0.1, and 1 mg/mL. Both extracts induced the proliferation of lymphocytes. However, PHA significantly (p< 0.05) stimulated the proliferation of lymphocytes about 204 % compared with hexane extract and 194 % as compared with methanol extract. Myrmecodia platytyrea extracts also showed concentration – dependant cytotoxicity on macrophage RAW 264.7. For hexane extract, the IC50 value was 6.7 ± 5.7 µg/ml with the maximum inhibition at 102.75 %. While for methanol extract, the IC50 value was 220.3 ± 53.5 µg/ml with the maximum inhibition at 165.06 %. In conclusion, Myrmecodia platytyrea hexane extract is more cytotoxic than methanol extract. On the other hand, both hexane and methanol extracts stimulated the proliferation of lymphocytes. However, methanol extract showed higher proliferative activity towards lymphocytes than hexane extract. It is believed that the proliferation activity of the Myrmecodia platytyrea extracts was due to the flavonoid and terpenoid compounds that contained in both extracts. 2012 Student Project NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/110010/1/110010.pdf Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni. (2012) [Student Project] <http://terminalib.uitm.edu.my/110010.pdf> (Unpublished)
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Immunology
spellingShingle Immunology
Jafni, Khadijah
Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni
description The in vitro study on immunology status is performed to determine the immunomodulatory effect of extracts on cell lines. The purpose of this study is to investigate the immunomodulatory response of Myrmecodia platytyrea hexane and methanol extracts toward Wistar rat’s lymphocytes and to study the cytotoxic effect of both extracts on macrophage RAW 264.7. Both experiments were using MTS assay to get rate of proliferation and rate of cell death. In determining the lymphocyte proliferation activity, mitogen such as PHA was used as a positive control. The concentrations of Myrmecodia platytyrea of both extracts used in this experiment are 0.0001, 0.001, 0.01, 0.1, and 1 mg/mL. Both extracts induced the proliferation of lymphocytes. However, PHA significantly (p< 0.05) stimulated the proliferation of lymphocytes about 204 % compared with hexane extract and 194 % as compared with methanol extract. Myrmecodia platytyrea extracts also showed concentration – dependant cytotoxicity on macrophage RAW 264.7. For hexane extract, the IC50 value was 6.7 ± 5.7 µg/ml with the maximum inhibition at 102.75 %. While for methanol extract, the IC50 value was 220.3 ± 53.5 µg/ml with the maximum inhibition at 165.06 %. In conclusion, Myrmecodia platytyrea hexane extract is more cytotoxic than methanol extract. On the other hand, both hexane and methanol extracts stimulated the proliferation of lymphocytes. However, methanol extract showed higher proliferative activity towards lymphocytes than hexane extract. It is believed that the proliferation activity of the Myrmecodia platytyrea extracts was due to the flavonoid and terpenoid compounds that contained in both extracts.
format Student Project
author Jafni, Khadijah
author_facet Jafni, Khadijah
author_sort Jafni, Khadijah
title Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni
title_short Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni
title_full Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni
title_fullStr Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni
title_full_unstemmed Effects of Myrmecodia platytyrea extract on immunology status (in vitro) / Khadijah Jafni
title_sort effects of myrmecodia platytyrea extract on immunology status (in vitro) / khadijah jafni
publishDate 2012
url https://ir.uitm.edu.my/id/eprint/110010/1/110010.pdf
https://ir.uitm.edu.my/id/eprint/110010/
_version_ 1823540802348908544
score 13.239859

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