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Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail

Lung cancer is among the five main types of cancer leading to overall cancer mortality contributing about 1.3 million deaths per year globally (Khurana, 2005). The factor that lead to the lung cancer to human is primarily due to bad habit of smoking which when there is a restriction of tobacco smoki...

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Main Author: Ismail, Nur Hidayah
Format: Thesis
Language:English
Published: 2013
Subjects:
Cancer
Online Access:https://ir.uitm.edu.my/id/eprint/109653/1/109653.PDF
https://ir.uitm.edu.my/id/eprint/109653/
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spelling my.uitm.ir.1096532025-01-31T23:53:23Z https://ir.uitm.edu.my/id/eprint/109653/ Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail Ismail, Nur Hidayah Cancer Lung cancer is among the five main types of cancer leading to overall cancer mortality contributing about 1.3 million deaths per year globally (Khurana, 2005). The factor that lead to the lung cancer to human is primarily due to bad habit of smoking which when there is a restriction of tobacco smoking can lower the possibility of lung cancer. The aim of the study is to examine the antioxidant and anti-proliferative effects of Gelam honey in lung cancer cell line A549 and also to determine any difference in gene expression of lung cancer that being treated with Gelam honey with the untreated lung cancer. Gelam honey becomes preference because antioxidant activity is significantly higher than the other honey due to its higher phenolic content. The antibacterial system of honey able to get rid the reactive hydroxyl radicals which under the control of antioxidant action of honey (Aljadi & Kamaruddin, 2004). Gene fishing kit was used to generate gene expression profiles between gelam honey treated A549 cells versus untreated cells. Unique bands were cloned using TOPO TA Cloning Kit and sequenced. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109653/1/109653.PDF Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
spellingShingle Cancer
Ismail, Nur Hidayah
Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail
description Lung cancer is among the five main types of cancer leading to overall cancer mortality contributing about 1.3 million deaths per year globally (Khurana, 2005). The factor that lead to the lung cancer to human is primarily due to bad habit of smoking which when there is a restriction of tobacco smoking can lower the possibility of lung cancer. The aim of the study is to examine the antioxidant and anti-proliferative effects of Gelam honey in lung cancer cell line A549 and also to determine any difference in gene expression of lung cancer that being treated with Gelam honey with the untreated lung cancer. Gelam honey becomes preference because antioxidant activity is significantly higher than the other honey due to its higher phenolic content. The antibacterial system of honey able to get rid the reactive hydroxyl radicals which under the control of antioxidant action of honey (Aljadi & Kamaruddin, 2004). Gene fishing kit was used to generate gene expression profiles between gelam honey treated A549 cells versus untreated cells. Unique bands were cloned using TOPO TA Cloning Kit and sequenced.
format Thesis
author Ismail, Nur Hidayah
author_facet Ismail, Nur Hidayah
author_sort Ismail, Nur Hidayah
title Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail
title_short Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail
title_full Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail
title_fullStr Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail
title_full_unstemmed Isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / Nur Hidayah Ismail
title_sort isolation of differentially regulated genes in lung cancer cell line by arbitrarily-primed reverse transcription polymerase chain reaction / nur hidayah ismail
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109653/1/109653.PDF
https://ir.uitm.edu.my/id/eprint/109653/
_version_ 1823097937727586304
score 13.235796

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