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In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib

During inflammation, reactive oxygen species (ROS) will be released into the injured site and cause further injury to the cells by initiating lipid peroxidation process. The action of ROS are limited by antioxidant enzymes. Antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) an...

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Main Author: Abd Mutalib, Nurhafizah
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109646/1/109646.PDF
https://ir.uitm.edu.my/id/eprint/109646/
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spelling my.uitm.ir.1096462025-02-13T23:55:02Z https://ir.uitm.edu.my/id/eprint/109646/ In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib Abd Mutalib, Nurhafizah Pharmacopoeias During inflammation, reactive oxygen species (ROS) will be released into the injured site and cause further injury to the cells by initiating lipid peroxidation process. The action of ROS are limited by antioxidant enzymes. Antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are produced in the body as body's defense against ROS to prevent them from damaging the body cells. This study was carried out to determine the anti-inflammatory properties of Erythroxylum cuneatum aqueous leaf extract in vitro by measuring the activity of superoxide dismutase, catalase and glutathione peroxidase on LPS­ induced RAW 264.7. Measurements of these enzymes were carried out using commercialized ELISA kits. Our findings showed that the aqueous leaf extract of E. cuneatum was able to inhibit the inflammation induced by LPS on RAW 264. 7. SOD and GPx levels were increased significantly in the cells compared to indomethacin. However, concentrations of catalase was not affected compared to the untreated LPS-induced cells. In conclusion, E.cuneatum extract contain anti-inflammatory properties through modulation of the activities of the antioxidant enzymes activities in the LPS-induced RAW 264.7. This further supports the folkloric claims of the medicinal values of the plant and showed its potential to be exploited in the nutraceutical industries. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109646/1/109646.PDF In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Abd Mutalib, Nurhafizah
In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib
description During inflammation, reactive oxygen species (ROS) will be released into the injured site and cause further injury to the cells by initiating lipid peroxidation process. The action of ROS are limited by antioxidant enzymes. Antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) are produced in the body as body's defense against ROS to prevent them from damaging the body cells. This study was carried out to determine the anti-inflammatory properties of Erythroxylum cuneatum aqueous leaf extract in vitro by measuring the activity of superoxide dismutase, catalase and glutathione peroxidase on LPS­ induced RAW 264.7. Measurements of these enzymes were carried out using commercialized ELISA kits. Our findings showed that the aqueous leaf extract of E. cuneatum was able to inhibit the inflammation induced by LPS on RAW 264. 7. SOD and GPx levels were increased significantly in the cells compared to indomethacin. However, concentrations of catalase was not affected compared to the untreated LPS-induced cells. In conclusion, E.cuneatum extract contain anti-inflammatory properties through modulation of the activities of the antioxidant enzymes activities in the LPS-induced RAW 264.7. This further supports the folkloric claims of the medicinal values of the plant and showed its potential to be exploited in the nutraceutical industries.
format Thesis
author Abd Mutalib, Nurhafizah
author_facet Abd Mutalib, Nurhafizah
author_sort Abd Mutalib, Nurhafizah
title In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib
title_short In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib
title_full In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib
title_fullStr In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib
title_full_unstemmed In vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in LPS-induced RAW264.7 / Nurhafizah Abd Mutalib
title_sort in vitro anti-inflammatory properties of aqueous erythroxylum cuneatum standardized extract on antioxidant enzymes in lps-induced raw264.7 / nurhafizah abd mutalib
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109646/1/109646.PDF
https://ir.uitm.edu.my/id/eprint/109646/
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score 13.239859

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