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Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali

Marine organisms possess unique structural features as compared to terrestrial organisms. The secondary metabolites produced by marine endophytic fungi have been regarded as an important source contributes to the natural chemotherapeutic agents. Due to the emergence of antibiotics resistance by cert...

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Main Author: Razali, Norasyikin
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:https://ir.uitm.edu.my/id/eprint/109607/1/109607.PDF
https://ir.uitm.edu.my/id/eprint/109607/
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spelling my.uitm.ir.1096072025-02-13T23:54:34Z https://ir.uitm.edu.my/id/eprint/109607/ Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali Razali, Norasyikin Pharmacopoeias Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) Marine organisms possess unique structural features as compared to terrestrial organisms. The secondary metabolites produced by marine endophytic fungi have been regarded as an important source contributes to the natural chemotherapeutic agents. Due to the emergence of antibiotics resistance by certain pathogenic bacteria, there is an urge to seek for new candidates for alternative drugs. In search for a new source of active metabolites, seaweeds have been studied for the past several years. In this study, five marine endophytic fungi isolated from four Malaysian brown seaweeds Cystoseira nudicaulis, Mesogloia vermiculata, Eucheuma denticulatum and Padina australis were investigated for the antimicrobial properties by using antibacterial assay and disc diffusion method. The endophytes were grown in two types of media, PDA and PDA with 3% artificial sea salt (PDA3%). Out of ten marine endophytic fungi extracts, eight extracts (CN PDA, CN PDA3%, MV PDA, MV PDA3%, EDl PDA, EDl PDA3%, ED2 PDA and PA7 PDA3%) exerted weak (growth inhibition <20%) to moderate (growth inhibition 2:37%) antibacterial activity against pathogenic bacteria B.subtilis, E.coli, P.aeruginosa, S.aureus, S.enterica and S.typhimurium. As for antifungal activity, eight extracts (CN PDA, CN PDA3%, MV PDA, MV PDA3%, EDl PDA, EDl PDA3%, ED2 PDA3%, PA7 PDA and PA7 PDA3%) exhibited zone inhibition in the range of 6.5±0.71 to 12.0±1.41 mm against pathogenic fungi A.niger, C.albicans and T.rubrum. Fungicidal activity of extracts CN and EDl (both from PDA3%) was observed at the minimal concentration tested (0.625 mg/ml). The antimicrobial activity strength of extracts CN, MY, EDl, ED2 and PA7 was influenced by certain factors such as chemical composition, salinity and concentration of extracts. Marine endophytic fungi extracts isolated from seaweeds of Cystoseira nudicaulis, Mesogloia vermiculata and Eucheuma denticulatum have a promising potential as an alternative source for antimicrobial drugs by inhibiting both of pathogenic bacteria and fungi growth. Further study should be carried out in order to have better understanding on their mechanism(s) of action and to identify the bioactive compounds which responsible for antimicrobial activities. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109607/1/109607.PDF Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle Pharmacopoeias
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Razali, Norasyikin
Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali
description Marine organisms possess unique structural features as compared to terrestrial organisms. The secondary metabolites produced by marine endophytic fungi have been regarded as an important source contributes to the natural chemotherapeutic agents. Due to the emergence of antibiotics resistance by certain pathogenic bacteria, there is an urge to seek for new candidates for alternative drugs. In search for a new source of active metabolites, seaweeds have been studied for the past several years. In this study, five marine endophytic fungi isolated from four Malaysian brown seaweeds Cystoseira nudicaulis, Mesogloia vermiculata, Eucheuma denticulatum and Padina australis were investigated for the antimicrobial properties by using antibacterial assay and disc diffusion method. The endophytes were grown in two types of media, PDA and PDA with 3% artificial sea salt (PDA3%). Out of ten marine endophytic fungi extracts, eight extracts (CN PDA, CN PDA3%, MV PDA, MV PDA3%, EDl PDA, EDl PDA3%, ED2 PDA and PA7 PDA3%) exerted weak (growth inhibition <20%) to moderate (growth inhibition 2:37%) antibacterial activity against pathogenic bacteria B.subtilis, E.coli, P.aeruginosa, S.aureus, S.enterica and S.typhimurium. As for antifungal activity, eight extracts (CN PDA, CN PDA3%, MV PDA, MV PDA3%, EDl PDA, EDl PDA3%, ED2 PDA3%, PA7 PDA and PA7 PDA3%) exhibited zone inhibition in the range of 6.5±0.71 to 12.0±1.41 mm against pathogenic fungi A.niger, C.albicans and T.rubrum. Fungicidal activity of extracts CN and EDl (both from PDA3%) was observed at the minimal concentration tested (0.625 mg/ml). The antimicrobial activity strength of extracts CN, MY, EDl, ED2 and PA7 was influenced by certain factors such as chemical composition, salinity and concentration of extracts. Marine endophytic fungi extracts isolated from seaweeds of Cystoseira nudicaulis, Mesogloia vermiculata and Eucheuma denticulatum have a promising potential as an alternative source for antimicrobial drugs by inhibiting both of pathogenic bacteria and fungi growth. Further study should be carried out in order to have better understanding on their mechanism(s) of action and to identify the bioactive compounds which responsible for antimicrobial activities.
format Thesis
author Razali, Norasyikin
author_facet Razali, Norasyikin
author_sort Razali, Norasyikin
title Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali
title_short Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali
title_full Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali
title_fullStr Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali
title_full_unstemmed Antimicrobial properties of marine endophytic fungi extracted from selected Malaysian sea weeds / Norasyikin Razali
title_sort antimicrobial properties of marine endophytic fungi extracted from selected malaysian sea weeds / norasyikin razali
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109607/1/109607.PDF
https://ir.uitm.edu.my/id/eprint/109607/
_version_ 1825165118469570560
score 13.239859

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