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The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad

The intestinal microbiota plays an important role m maintaining host health. Bifidobacteria which inhabit gastrointestinal tracts (GIT) has antimicrobial properties that maintains gut function and contributes to the GIT defense system. Therefore, prebiotic as non-digestible carbohydrate with the abi...

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Main Author: Saad, Nurul Akmal
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Online Access:https://ir.uitm.edu.my/id/eprint/109604/1/109604.PDF
https://ir.uitm.edu.my/id/eprint/109604/
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spelling my.uitm.ir.1096042025-01-31T23:53:45Z https://ir.uitm.edu.my/id/eprint/109604/ The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad Saad, Nurul Akmal Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) The intestinal microbiota plays an important role m maintaining host health. Bifidobacteria which inhabit gastrointestinal tracts (GIT) has antimicrobial properties that maintains gut function and contributes to the GIT defense system. Therefore, prebiotic as non-digestible carbohydrate with the ability to stimulate and enhance the growth of bifidobacteria has evoked great interest among researchers. Honey serves as potential sources of prebiotic. This study was carried out to investigate the potential prebiotic effect of Malaysian honey namely Gelam and Tualang to enhance the growth and activity of Bifidobacterium adolescentis ATCC 15705, a probiotic in modified De Man Rogosa Sharpe (MRS) media. The growth promoting effects of both honeys were compared with commercial fructooligosaccharide (FOS). Inoculated samples were incubated anaerobically at 37 °C for 48 h. Samples were collected at 0, 24 and 48 h incubation and examined for specific growth rate, viability of bacteria and level of fermentation (acid and lactic acid production) as measured by high pressure liquid chromatography (HPLC). The results showed that, both honey enhanced (p<0.05) the growth and activity of B.adolescentis. The production of lactic acid was enhanced (p<0.05) when bifidobacteria was grown in the presence of Gelam and Tualang honey compared with FOS. The results obtained demonstrated that both types of local honey supported the growth and activity of B.adolescentis better than FOS. However, Gelam honey 5% (w/v) showed the highest prebiotic effect compared to Tualang honey. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109604/1/109604.PDF The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
spellingShingle Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Saad, Nurul Akmal
The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad
description The intestinal microbiota plays an important role m maintaining host health. Bifidobacteria which inhabit gastrointestinal tracts (GIT) has antimicrobial properties that maintains gut function and contributes to the GIT defense system. Therefore, prebiotic as non-digestible carbohydrate with the ability to stimulate and enhance the growth of bifidobacteria has evoked great interest among researchers. Honey serves as potential sources of prebiotic. This study was carried out to investigate the potential prebiotic effect of Malaysian honey namely Gelam and Tualang to enhance the growth and activity of Bifidobacterium adolescentis ATCC 15705, a probiotic in modified De Man Rogosa Sharpe (MRS) media. The growth promoting effects of both honeys were compared with commercial fructooligosaccharide (FOS). Inoculated samples were incubated anaerobically at 37 °C for 48 h. Samples were collected at 0, 24 and 48 h incubation and examined for specific growth rate, viability of bacteria and level of fermentation (acid and lactic acid production) as measured by high pressure liquid chromatography (HPLC). The results showed that, both honey enhanced (p<0.05) the growth and activity of B.adolescentis. The production of lactic acid was enhanced (p<0.05) when bifidobacteria was grown in the presence of Gelam and Tualang honey compared with FOS. The results obtained demonstrated that both types of local honey supported the growth and activity of B.adolescentis better than FOS. However, Gelam honey 5% (w/v) showed the highest prebiotic effect compared to Tualang honey.
format Thesis
author Saad, Nurul Akmal
author_facet Saad, Nurul Akmal
author_sort Saad, Nurul Akmal
title The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad
title_short The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad
title_full The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad
title_fullStr The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad
title_full_unstemmed The effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / Nurul Akmal Saad
title_sort effects of gelam and tualang honey on the growth and activity of bifidobacterium adolescentis / nurul akmal saad
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109604/1/109604.PDF
https://ir.uitm.edu.my/id/eprint/109604/
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score 13.235796

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