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The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman

Recombinant E. coli is seen here as an alternative toward production of phospholipase A2 enzymes. The recombinant PLA2 of pBADTOPO-pla2 5 and pBADTOPO-pla2 8 were successfully cloned and sequence in previous study. The clones were recognized using SDS-PAGE and the result found that the recombinant P...

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Main Author: Abd Rahman, Zainab
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109583/1/109583.PDF
https://ir.uitm.edu.my/id/eprint/109583/
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spelling my.uitm.ir.1095832025-02-13T23:54:31Z https://ir.uitm.edu.my/id/eprint/109583/ The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman Abd Rahman, Zainab Pharmacopoeias Recombinant E. coli is seen here as an alternative toward production of phospholipase A2 enzymes. The recombinant PLA2 of pBADTOPO-pla2 5 and pBADTOPO-pla2 8 were successfully cloned and sequence in previous study. The clones were recognized using SDS-PAGE and the result found that the recombinant PLA2 was successfully expressed as a soluble protein by arabinose induction. Study on kinetics growth of recombinant PLA2 creates more understanding on their biological activity in which can be used for future optimization in fermentation process. The kinetics growth of recombinant PLA2 was study in shake flask culture and their growth pattern was observed through cells viability, Bradford protein assays and dry cell weight. The culture was grown using suitable medium with suitable growth condition. The catalytic activity of recombinant pBADTOPO-pla2 5 and pBADTOPO-pla2 8 was then tested through sPLA2 assay and found to display enzymatic activity. Results from kinetics study reveal that the recombinant proteins shown normal growth pattern with high protein production at log phase. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109583/1/109583.PDF The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Abd Rahman, Zainab
The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman
description Recombinant E. coli is seen here as an alternative toward production of phospholipase A2 enzymes. The recombinant PLA2 of pBADTOPO-pla2 5 and pBADTOPO-pla2 8 were successfully cloned and sequence in previous study. The clones were recognized using SDS-PAGE and the result found that the recombinant PLA2 was successfully expressed as a soluble protein by arabinose induction. Study on kinetics growth of recombinant PLA2 creates more understanding on their biological activity in which can be used for future optimization in fermentation process. The kinetics growth of recombinant PLA2 was study in shake flask culture and their growth pattern was observed through cells viability, Bradford protein assays and dry cell weight. The culture was grown using suitable medium with suitable growth condition. The catalytic activity of recombinant pBADTOPO-pla2 5 and pBADTOPO-pla2 8 was then tested through sPLA2 assay and found to display enzymatic activity. Results from kinetics study reveal that the recombinant proteins shown normal growth pattern with high protein production at log phase.
format Thesis
author Abd Rahman, Zainab
author_facet Abd Rahman, Zainab
author_sort Abd Rahman, Zainab
title The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman
title_short The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman
title_full The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman
title_fullStr The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman
title_full_unstemmed The kinetic growth study of escherichia coli in production recombinant PLA2 / Zainab Abd Rahman
title_sort kinetic growth study of escherichia coli in production recombinant pla2 / zainab abd rahman
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109583/1/109583.PDF
https://ir.uitm.edu.my/id/eprint/109583/
_version_ 1825165118136123392
score 13.239859

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