Cover Image

Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman

Klebsiella pneumoniae is the gram-negative bacteria that most frequently encountered by the physician worldwide due to their ability to cause nosocomial infections such as nosocomial pneumoma, urinary tract infections, and intraabdominal infections. It is also a potential community-acquired pathogen...

Full description

Saved in:
Bibliographic Details
Main Author: Azman, Rabiatul Adawiyah
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109552/1/109552.PDF
https://ir.uitm.edu.my/id/eprint/109552/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.uitm.ir.109552
record_format eprints
spelling my.uitm.ir.1095522025-02-14T12:44:38Z https://ir.uitm.edu.my/id/eprint/109552/ Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman Azman, Rabiatul Adawiyah Pharmacopoeias Klebsiella pneumoniae is the gram-negative bacteria that most frequently encountered by the physician worldwide due to their ability to cause nosocomial infections such as nosocomial pneumoma, urinary tract infections, and intraabdominal infections. It is also a potential community-acquired pathogen causing primary liver abscesses, metastatic meningitis and endophthalmitis. The laboratory identification of these bacteria may be time-consuming. So, the purpose of this study was to develop a Polymerase Chain Reaction method for the rapid identification of the K. pneumoniae which is critical for the definitive diagnosis of the disease caused by those bacteria. Four pairs of primer have been designed to aid with the identification of these bacteria through the selected genes. The detection using PCR has revealed that K. pneumoniae was specific to those selected genes because the bands from gel electrophoresis of PCR products appeared when viewed under UV. Thus, PCR method developed is successful in detecting the Klebsiella pneumoniae rapidly and specifically. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109552/1/109552.PDF Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Azman, Rabiatul Adawiyah
Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman
description Klebsiella pneumoniae is the gram-negative bacteria that most frequently encountered by the physician worldwide due to their ability to cause nosocomial infections such as nosocomial pneumoma, urinary tract infections, and intraabdominal infections. It is also a potential community-acquired pathogen causing primary liver abscesses, metastatic meningitis and endophthalmitis. The laboratory identification of these bacteria may be time-consuming. So, the purpose of this study was to develop a Polymerase Chain Reaction method for the rapid identification of the K. pneumoniae which is critical for the definitive diagnosis of the disease caused by those bacteria. Four pairs of primer have been designed to aid with the identification of these bacteria through the selected genes. The detection using PCR has revealed that K. pneumoniae was specific to those selected genes because the bands from gel electrophoresis of PCR products appeared when viewed under UV. Thus, PCR method developed is successful in detecting the Klebsiella pneumoniae rapidly and specifically.
format Thesis
author Azman, Rabiatul Adawiyah
author_facet Azman, Rabiatul Adawiyah
author_sort Azman, Rabiatul Adawiyah
title Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman
title_short Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman
title_full Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman
title_fullStr Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman
title_full_unstemmed Detection of klebsiella pneumoniae using polymerase chain reaction (PCR) / Rabiatul Adawiyah Azman
title_sort detection of klebsiella pneumoniae using polymerase chain reaction (pcr) / rabiatul adawiyah azman
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109552/1/109552.PDF
https://ir.uitm.edu.my/id/eprint/109552/
_version_ 1825165117300408320
score 13.239859

Similar Items