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Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman

miRNA is a small non-coding RNA region that is not translated to protein. This region usually involved in the regulation and expression the cell. Many studies were done on the miRNA and showed high correlation with certain cancer disease such as liver cancer. The aim of the study is to clone the miR...

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Main Author: Abd Rahman, Nazihah
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109537/1/109537.PDF
https://ir.uitm.edu.my/id/eprint/109537/
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spelling my.uitm.ir.1095372025-02-14T11:55:19Z https://ir.uitm.edu.my/id/eprint/109537/ Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman Abd Rahman, Nazihah Pharmacopoeias miRNA is a small non-coding RNA region that is not translated to protein. This region usually involved in the regulation and expression the cell. Many studies were done on the miRNA and showed high correlation with certain cancer disease such as liver cancer. The aim of the study is to clone the miRNA -17 -l 8a of HepG2 cancer cell line in a vector. Initially, the region was amplified using pair of primer designed based on the sequences of the miRNA -17 -18a region fron GenBank. Optimum annealing temperature obtained from the PCR optimization was 51 °c. The PCR product was then ligated into the plasmid based on TA cloning method. The presence of insert and plasmid into the competent cell was confirmed using blue white screening method. Besides that, PCR cloning using Ml3 universal primer was done to further prove the presence of insert in the plasmid vector. Nevertheless, based on the result of sequencing, the inserted region was not the desired region as it did not aligned with the sequence of miRNAl 7 and 18a from NCBI. This might due to the unspecific binding of the primer pair designed earlier. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109537/1/109537.PDF Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Abd Rahman, Nazihah
Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman
description miRNA is a small non-coding RNA region that is not translated to protein. This region usually involved in the regulation and expression the cell. Many studies were done on the miRNA and showed high correlation with certain cancer disease such as liver cancer. The aim of the study is to clone the miRNA -17 -l 8a of HepG2 cancer cell line in a vector. Initially, the region was amplified using pair of primer designed based on the sequences of the miRNA -17 -18a region fron GenBank. Optimum annealing temperature obtained from the PCR optimization was 51 °c. The PCR product was then ligated into the plasmid based on TA cloning method. The presence of insert and plasmid into the competent cell was confirmed using blue white screening method. Besides that, PCR cloning using Ml3 universal primer was done to further prove the presence of insert in the plasmid vector. Nevertheless, based on the result of sequencing, the inserted region was not the desired region as it did not aligned with the sequence of miRNAl 7 and 18a from NCBI. This might due to the unspecific binding of the primer pair designed earlier.
format Thesis
author Abd Rahman, Nazihah
author_facet Abd Rahman, Nazihah
author_sort Abd Rahman, Nazihah
title Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman
title_short Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman
title_full Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman
title_fullStr Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman
title_full_unstemmed Cloning and sequencing of microRNA-17 and -18a genes from HepG2 cancer cell line / Nazihah Abd Rahman
title_sort cloning and sequencing of microrna-17 and -18a genes from hepg2 cancer cell line / nazihah abd rahman
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109537/1/109537.PDF
https://ir.uitm.edu.my/id/eprint/109537/
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score 13.239859

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