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Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom

Acinetobacter baumannii is a multidrug resistant bacterium and a major pathogen causing nosocomial infections worldwide, including pneumonia, wound infections, urinary tract infections, bacteremia and meningitis. A rapid, sensitive and specific detection method is needed for cases involving Aibauman...

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Main Author: Shahrom, Noradila
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109501/1/109501.PDF
https://ir.uitm.edu.my/id/eprint/109501/
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spelling my.uitm.ir.1095012025-02-13T23:58:18Z https://ir.uitm.edu.my/id/eprint/109501/ Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom Shahrom, Noradila Pharmacopoeias Acinetobacter baumannii is a multidrug resistant bacterium and a major pathogen causing nosocomial infections worldwide, including pneumonia, wound infections, urinary tract infections, bacteremia and meningitis. A rapid, sensitive and specific detection method is needed for cases involving Aibaumannii. Thus, the objective of this study is to develop a diagnostic test, which is PCR for the detection of Aibaumannii. The primers were designed for Conserved gene 1, Conserved gene 2 and ada[H] gene. Results showed that the detection of those genes was successful. Through visualization of gel electrophoresis result for PCR products, DNA bands for Conserved gene 1, Conserved gene 2, ada[H] gene and multiplex PCR were observed. This showed that those genes were present in Abaumannii and were successfully detected using the developed diagnostic test. The detection of A.baumannii using the novel primers was successful. PCR assay can be applied as a robust, rapid, sensitive and specific detection method for Abaumannii. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109501/1/109501.PDF Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Shahrom, Noradila
Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
description Acinetobacter baumannii is a multidrug resistant bacterium and a major pathogen causing nosocomial infections worldwide, including pneumonia, wound infections, urinary tract infections, bacteremia and meningitis. A rapid, sensitive and specific detection method is needed for cases involving Aibaumannii. Thus, the objective of this study is to develop a diagnostic test, which is PCR for the detection of Aibaumannii. The primers were designed for Conserved gene 1, Conserved gene 2 and ada[H] gene. Results showed that the detection of those genes was successful. Through visualization of gel electrophoresis result for PCR products, DNA bands for Conserved gene 1, Conserved gene 2, ada[H] gene and multiplex PCR were observed. This showed that those genes were present in Abaumannii and were successfully detected using the developed diagnostic test. The detection of A.baumannii using the novel primers was successful. PCR assay can be applied as a robust, rapid, sensitive and specific detection method for Abaumannii.
format Thesis
author Shahrom, Noradila
author_facet Shahrom, Noradila
author_sort Shahrom, Noradila
title Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
title_short Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
title_full Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
title_fullStr Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
title_full_unstemmed Detection of acinetobacter baumannii using polymerase chain reaction / Noradila Shahrom
title_sort detection of acinetobacter baumannii using polymerase chain reaction / noradila shahrom
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109501/1/109501.PDF
https://ir.uitm.edu.my/id/eprint/109501/
_version_ 1825165113477300224
score 13.239859

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