Cover Image

In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof

Erythroxylum cuneatum has long been used by the folks as a traditional herbal medicine to treat inflammatory disease such as rheumatoid arthritis by chewing the dried leaves. This plant was chosen because only few studied was conducted on Erythroxylum cuneatum. The aim of the study is to determine t...

Full description

Saved in:
Bibliographic Details
Main Author: Maarof, Siti Khuzaimah
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109405/1/109405.PDF
https://ir.uitm.edu.my/id/eprint/109405/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.uitm.ir.109405
record_format eprints
spelling my.uitm.ir.1094052025-02-14T11:48:53Z https://ir.uitm.edu.my/id/eprint/109405/ In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof Maarof, Siti Khuzaimah Pharmacopoeias Erythroxylum cuneatum has long been used by the folks as a traditional herbal medicine to treat inflammatory disease such as rheumatoid arthritis by chewing the dried leaves. This plant was chosen because only few studied was conducted on Erythroxylum cuneatum. The aim of the study is to determine the in-vitro anti­ inflammatory activity of standardized aqueous extract of Erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells. We evaluate the protective and toxicity effect of Erythroxylum cuneatum by measuring the percent cell death in MIT assay. The pro-inflammatory cytokines such as Tumor Necrosis Factor alpha (TNF-a) and Interleukin- I a (IL- I a) production were evaluated after the cell were induced by the lipopolysaccharide (LPS) of gram negative bacteria with presence of Erythroxylum cuneatum. The pro-inflammatory cytokine were evaluated by ELISA. In this research, upon stimulation of Erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cell showed that there are significantly reduced of the amount of pro-inflammatory cytokines production. These results suggest that Erythroxylum cuneatum may have anti­ inflammatory activity by inhibiting TNF-a and IL-1 a in RAW 264. 7 cell. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109405/1/109405.PDF In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Maarof, Siti Khuzaimah
In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof
description Erythroxylum cuneatum has long been used by the folks as a traditional herbal medicine to treat inflammatory disease such as rheumatoid arthritis by chewing the dried leaves. This plant was chosen because only few studied was conducted on Erythroxylum cuneatum. The aim of the study is to determine the in-vitro anti­ inflammatory activity of standardized aqueous extract of Erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells. We evaluate the protective and toxicity effect of Erythroxylum cuneatum by measuring the percent cell death in MIT assay. The pro-inflammatory cytokines such as Tumor Necrosis Factor alpha (TNF-a) and Interleukin- I a (IL- I a) production were evaluated after the cell were induced by the lipopolysaccharide (LPS) of gram negative bacteria with presence of Erythroxylum cuneatum. The pro-inflammatory cytokine were evaluated by ELISA. In this research, upon stimulation of Erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cell showed that there are significantly reduced of the amount of pro-inflammatory cytokines production. These results suggest that Erythroxylum cuneatum may have anti­ inflammatory activity by inhibiting TNF-a and IL-1 a in RAW 264. 7 cell.
format Thesis
author Maarof, Siti Khuzaimah
author_facet Maarof, Siti Khuzaimah
author_sort Maarof, Siti Khuzaimah
title In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof
title_short In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof
title_full In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof
title_fullStr In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof
title_full_unstemmed In-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced RAW 264.7 cells / Siti Khuzaimah Maarof
title_sort in-vitro anti-inflammatory activity of standardized aqueous extract of erythroxylum cuneatum in lipopolysaccharide-induced raw 264.7 cells / siti khuzaimah maarof
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109405/1/109405.PDF
https://ir.uitm.edu.my/id/eprint/109405/
_version_ 1825165111588814848
score 13.239859

Similar Items