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Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad

Paclitaxel is one of the most important drug of choice for cancer treatment. However, commercially available paclitaxel used in the current clinical setting is formulated in Cremophor EL, a solvent which has been reported to elicit a number of adverse side effects. Nanoparticle (NP) technology may p...

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Main Author: Ahmad, Nurul Bazliah
Format: Thesis
Language:English
Published: 2013
Subjects:
Drugs prescribing
Drugs and their actions
Online Access:https://ir.uitm.edu.my/id/eprint/109389/1/109389.PDF
https://ir.uitm.edu.my/id/eprint/109389/
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spelling my.uitm.ir.1093892025-01-23T15:16:52Z https://ir.uitm.edu.my/id/eprint/109389/ Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad Ahmad, Nurul Bazliah Drugs prescribing Drugs and their actions Paclitaxel is one of the most important drug of choice for cancer treatment. However, commercially available paclitaxel used in the current clinical setting is formulated in Cremophor EL, a solvent which has been reported to elicit a number of adverse side effects. Nanoparticle (NP) technology may provide a safer alternative for sustained chemotherapy. The aim of this work was to investigate the efficiency of cellular uptake of polymeric nanoparticles which were coated with poly methacrylic acid (PMAA), poly acrylic acid (PAA), and poly N-vinyl caprolactam (PNVCL) using an in vitro model (breast cancer triple negative MDA-231 cells). Coumarin-6 loaded polymeric nanoparticles were prepared by polymerization dispersion method. Coumarin-6 was used for the purpose of fluorescent labelling to assist in the detection of cellular uptake of nanoparticles by MDA-231 cells. In the present study, the use of fluorescence plate reader was utilized in quantifying the efficiency of cell uptake. Cellular uptake of coumarin-6 loaded nanoparticles by MDA-231 was highest (8.20%) in poly N-vinyl caprolactam (PNVCL), when compared to poly methacrylic acid (PMAA, 7.55%) or poly acrylic acid (PAA, 6.23%) nanoparticles. The PNVCL coated nanoparticles showed greater advantages in vitro compared to the other two formulations. Studies on PNVCL coated nanoparticles deserve further attention and more studies on its efficacy are important. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109389/1/109389.PDF Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Drugs prescribing
Drugs and their actions
spellingShingle Drugs prescribing
Drugs and their actions
Ahmad, Nurul Bazliah
Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad
description Paclitaxel is one of the most important drug of choice for cancer treatment. However, commercially available paclitaxel used in the current clinical setting is formulated in Cremophor EL, a solvent which has been reported to elicit a number of adverse side effects. Nanoparticle (NP) technology may provide a safer alternative for sustained chemotherapy. The aim of this work was to investigate the efficiency of cellular uptake of polymeric nanoparticles which were coated with poly methacrylic acid (PMAA), poly acrylic acid (PAA), and poly N-vinyl caprolactam (PNVCL) using an in vitro model (breast cancer triple negative MDA-231 cells). Coumarin-6 loaded polymeric nanoparticles were prepared by polymerization dispersion method. Coumarin-6 was used for the purpose of fluorescent labelling to assist in the detection of cellular uptake of nanoparticles by MDA-231 cells. In the present study, the use of fluorescence plate reader was utilized in quantifying the efficiency of cell uptake. Cellular uptake of coumarin-6 loaded nanoparticles by MDA-231 was highest (8.20%) in poly N-vinyl caprolactam (PNVCL), when compared to poly methacrylic acid (PMAA, 7.55%) or poly acrylic acid (PAA, 6.23%) nanoparticles. The PNVCL coated nanoparticles showed greater advantages in vitro compared to the other two formulations. Studies on PNVCL coated nanoparticles deserve further attention and more studies on its efficacy are important.
format Thesis
author Ahmad, Nurul Bazliah
author_facet Ahmad, Nurul Bazliah
author_sort Ahmad, Nurul Bazliah
title Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad
title_short Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad
title_full Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad
title_fullStr Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad
title_full_unstemmed Study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (MDA-231) / Nurul Bazliah Ahmad
title_sort study of cellular uptake of novel drug formulations for paclitaxel on triple negative breast cancer cells (mda-231) / nurul bazliah ahmad
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109389/1/109389.PDF
https://ir.uitm.edu.my/id/eprint/109389/
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score 13.235796

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