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Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik

Angiotensin converting enzyme (ACE) is an enzyme responsible for the conversion of angiotensin I to angiotensin II and degradation of bradykinin. Polymorphism of ACE means that there is variation in the nucleotide sequence of ACE gene due to insertion (I) or deletion (D). There are several types of...

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Main Author: Abdul Malik, Nurul Fazleen
Format: Thesis
Language:English
Published: 2013
Subjects:
Animal biochemistry
Online Access:https://ir.uitm.edu.my/id/eprint/109256/1/109256.PDF
https://ir.uitm.edu.my/id/eprint/109256/
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spelling my.uitm.ir.1092562025-01-19T04:31:59Z https://ir.uitm.edu.my/id/eprint/109256/ Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik Abdul Malik, Nurul Fazleen Animal biochemistry Angiotensin converting enzyme (ACE) is an enzyme responsible for the conversion of angiotensin I to angiotensin II and degradation of bradykinin. Polymorphism of ACE means that there is variation in the nucleotide sequence of ACE gene due to insertion (I) or deletion (D). There are several types of polymorphism of ACE, in which it may give different responses whether by enhancing or reducing the efficiency of ACE inhibitor. The aim of this study is to develop a PCR method to detect polymorphism of ACE gene. The primer were designed according to the gene and followed by reconstitution of primer working stock. DNA sensitivity was performed in order to detect the lowest concentration of DNA template that can be used for PCR method. Besides that, different DNA of patient was used in order to detect polymorphism of ACE by using this PCR method. Based on the result of this study, it can be concluded that VD polymorphism of ACE gene was not successfully amplified by using the PCR. It is suggested that for next studies to used Restriction Fragment Length Polymorphism (RFLP) method for more accurate results. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109256/1/109256.PDF Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Animal biochemistry
spellingShingle Animal biochemistry
Abdul Malik, Nurul Fazleen
Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik
description Angiotensin converting enzyme (ACE) is an enzyme responsible for the conversion of angiotensin I to angiotensin II and degradation of bradykinin. Polymorphism of ACE means that there is variation in the nucleotide sequence of ACE gene due to insertion (I) or deletion (D). There are several types of polymorphism of ACE, in which it may give different responses whether by enhancing or reducing the efficiency of ACE inhibitor. The aim of this study is to develop a PCR method to detect polymorphism of ACE gene. The primer were designed according to the gene and followed by reconstitution of primer working stock. DNA sensitivity was performed in order to detect the lowest concentration of DNA template that can be used for PCR method. Besides that, different DNA of patient was used in order to detect polymorphism of ACE by using this PCR method. Based on the result of this study, it can be concluded that VD polymorphism of ACE gene was not successfully amplified by using the PCR. It is suggested that for next studies to used Restriction Fragment Length Polymorphism (RFLP) method for more accurate results.
format Thesis
author Abdul Malik, Nurul Fazleen
author_facet Abdul Malik, Nurul Fazleen
author_sort Abdul Malik, Nurul Fazleen
title Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik
title_short Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik
title_full Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik
title_fullStr Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik
title_full_unstemmed Development of a PCR method to detect polymorphism of angiotensin converting enzyme / Nurul Fazleen Abdul Malik
title_sort development of a pcr method to detect polymorphism of angiotensin converting enzyme / nurul fazleen abdul malik
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109256/1/109256.PDF
https://ir.uitm.edu.my/id/eprint/109256/
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score 13.244413

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