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Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan

Cancer is still a major public health problem around the globe with current therapies which lack selectivity and account for the other serious side effects. Plants play important roles as remedies even from centuries ago and plant derived secondary metabolites possess anticancer property. The in vit...

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Main Author: Azlan, Safia
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Pharmaceutical chemistry
Online Access:https://ir.uitm.edu.my/id/eprint/109204/1/109204.PDF
https://ir.uitm.edu.my/id/eprint/109204/
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spelling my.uitm.ir.1092042025-02-21T23:45:17Z https://ir.uitm.edu.my/id/eprint/109204/ Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan Azlan, Safia Pharmacopoeias Pharmaceutical chemistry Cancer is still a major public health problem around the globe with current therapies which lack selectivity and account for the other serious side effects. Plants play important roles as remedies even from centuries ago and plant derived secondary metabolites possess anticancer property. The in vitro anticancer activity of the methanol extract of the Clinacanthus nutans, Mentha piperita and seeds of Nigel/a sativa were evaluated against liver hepatocellular carcinoma (HepG2) and Chang liver cell lines. The fresh leaves of C. nutans, M piperita and the seeds of N sativa were air-dried and then soaked with methanol in ratio of 1 :20 (w/v) for 72 hours followed by subsequent evaporation to dryness process. The crude dried extracts were prepared in the concentration of 10 mg/ml by redissolving them in dimethyl sulfoxide (DMSO) and the extract were tested on the HepG2 and Chang liver cell line which then subjected to the 3,(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MIT) assay for anticancer activity. Based on the results, M piperita extracts showed the best anticancer activity against the HepG2 cell line which followed by C. nutans extract and seeds of N sativa extract with IC50 of 9.9 µg/ml, 10.0 µg/ml and 66.0 µg/ml respectively. IC50 was not observed in any of the extracts when tested on Chang liver cell line indicates selectivity only to cancer cells but not normal cells. It can be concluded that methanol extract of all of the plants contain active ingredient with potential selective anticancer property. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109204/1/109204.PDF Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
Pharmaceutical chemistry
spellingShingle Pharmacopoeias
Pharmaceutical chemistry
Azlan, Safia
Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan
description Cancer is still a major public health problem around the globe with current therapies which lack selectivity and account for the other serious side effects. Plants play important roles as remedies even from centuries ago and plant derived secondary metabolites possess anticancer property. The in vitro anticancer activity of the methanol extract of the Clinacanthus nutans, Mentha piperita and seeds of Nigel/a sativa were evaluated against liver hepatocellular carcinoma (HepG2) and Chang liver cell lines. The fresh leaves of C. nutans, M piperita and the seeds of N sativa were air-dried and then soaked with methanol in ratio of 1 :20 (w/v) for 72 hours followed by subsequent evaporation to dryness process. The crude dried extracts were prepared in the concentration of 10 mg/ml by redissolving them in dimethyl sulfoxide (DMSO) and the extract were tested on the HepG2 and Chang liver cell line which then subjected to the 3,(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MIT) assay for anticancer activity. Based on the results, M piperita extracts showed the best anticancer activity against the HepG2 cell line which followed by C. nutans extract and seeds of N sativa extract with IC50 of 9.9 µg/ml, 10.0 µg/ml and 66.0 µg/ml respectively. IC50 was not observed in any of the extracts when tested on Chang liver cell line indicates selectivity only to cancer cells but not normal cells. It can be concluded that methanol extract of all of the plants contain active ingredient with potential selective anticancer property.
format Thesis
author Azlan, Safia
author_facet Azlan, Safia
author_sort Azlan, Safia
title Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan
title_short Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan
title_full Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan
title_fullStr Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan
title_full_unstemmed Investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.F.), mentha piperita (Linn.) and seeds of nigella sativa (Linn.) on HePG2 and chang liver cell line / Safia Azlan
title_sort investigation on the cytotoxic effects of the methanol extract of clinacanthus nutans (burm.f.), mentha piperita (linn.) and seeds of nigella sativa (linn.) on hepg2 and chang liver cell line / safia azlan
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109204/1/109204.PDF
https://ir.uitm.edu.my/id/eprint/109204/
_version_ 1825165108802748416
score 13.239859

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