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Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth

Lung cancer has become one of the most deadly cancers in the world. One of the main causes of cancer is reactive oxygen species (ROS) in which it causes the increase of oxidant as compared to antioxidant. This imbalance can contribute to cancer pathogenesis. This study aims to know the differentiall...

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Main Author: Mohammad Seth, Siti Nazurah
Format: Thesis
Language:English
Published: 2013
Subjects:
Cancer
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109202/1/109202.PDF
https://ir.uitm.edu.my/id/eprint/109202/
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spelling my.uitm.ir.1092022025-02-18T17:47:09Z https://ir.uitm.edu.my/id/eprint/109202/ Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth Mohammad Seth, Siti Nazurah Cancer Pharmacopoeias Lung cancer has become one of the most deadly cancers in the world. One of the main causes of cancer is reactive oxygen species (ROS) in which it causes the increase of oxidant as compared to antioxidant. This imbalance can contribute to cancer pathogenesis. This study aims to know the differentially express gene of lung cancer cell line, Human Lung Adenocarcinoma Epithelial Cell Line 549 (A 549) when being treated with Gelam honey. Gelam honey is chosen in this research as it contains high amount of flavonoid and phenolic compounds which act as antioxidants and free radical scavenger. The amplification of lung cancer cell line A 549 by using GeneFishing™ DEG Premix Kit shows the down-regulated gene of cell treated with Gelam honey as compared to the untreated cell. The gene was successfully clone by using TOPO TA Cloning® Kit for Sequencing to identify the differentially express gene. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109202/1/109202.PDF Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Cancer
Pharmacopoeias
spellingShingle Cancer
Pharmacopoeias
Mohammad Seth, Siti Nazurah
Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth
description Lung cancer has become one of the most deadly cancers in the world. One of the main causes of cancer is reactive oxygen species (ROS) in which it causes the increase of oxidant as compared to antioxidant. This imbalance can contribute to cancer pathogenesis. This study aims to know the differentially express gene of lung cancer cell line, Human Lung Adenocarcinoma Epithelial Cell Line 549 (A 549) when being treated with Gelam honey. Gelam honey is chosen in this research as it contains high amount of flavonoid and phenolic compounds which act as antioxidants and free radical scavenger. The amplification of lung cancer cell line A 549 by using GeneFishing™ DEG Premix Kit shows the down-regulated gene of cell treated with Gelam honey as compared to the untreated cell. The gene was successfully clone by using TOPO TA Cloning® Kit for Sequencing to identify the differentially express gene.
format Thesis
author Mohammad Seth, Siti Nazurah
author_facet Mohammad Seth, Siti Nazurah
author_sort Mohammad Seth, Siti Nazurah
title Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth
title_short Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth
title_full Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth
title_fullStr Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth
title_full_unstemmed Identification of differentially expressed genes in a lung cancer cell line, A549 treated with gelam honey / Siti Nazurah Mohammad Seth
title_sort identification of differentially expressed genes in a lung cancer cell line, a549 treated with gelam honey / siti nazurah mohammad seth
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109202/1/109202.PDF
https://ir.uitm.edu.my/id/eprint/109202/
_version_ 1825165108644413440
score 13.239859

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