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Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil

Lactobacillus plantarum (L. plantarum) is one of the probiotics that widely studied since the fact that, it has copious potential health benefits. In this study, growth L. plantarum was studied by addition of prebiotics and non prebiotics such as com starch, gum Arabic and glucose. The fermentation...

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Main Author: Awang Jalil, Nora
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109156/1/109156.PDF
https://ir.uitm.edu.my/id/eprint/109156/
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spelling my.uitm.ir.1091562025-02-18T18:04:50Z https://ir.uitm.edu.my/id/eprint/109156/ Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil Awang Jalil, Nora Pharmacopoeias Lactobacillus plantarum (L. plantarum) is one of the probiotics that widely studied since the fact that, it has copious potential health benefits. In this study, growth L. plantarum was studied by addition of prebiotics and non prebiotics such as com starch, gum Arabic and glucose. The fermentation of L. plantarum was done in shake flasks and incubated aerobically at 37°C for 24 hours and the result was measured by optical density at 620nm wavelength (OD620) using spectrophotometer. The fermentation media was prepared for 15 runs with different concentrations of prebiotics and non-prebiotic by following three factorial Box-Behnken designs. The principle of this study is to screen prebiotics by investigate the main and interaction effect on the cell growth of L. plantarum. In this experiment, L. plantarum fermentation revealed growth patterns which include lag phase, exponential phase, stationary phase and death phase. Based on the results, maximal optical density of L. plantarum was 0.226 OD620 which contain high proportion of com starch. However, referring to specific growth rate of L. plantarum, it was high when the maximum presence of gum Arabic compared to com starch. For further results, analysis of variance (ANOVA) indicated that all prebiotics and · non-prebiotic had no significance difference (p>0.05) on growth capability of this strain. Response surface methodology (RSM) predicted optimum concentrations of prebiotics, only concentration of gum Arabic and glucose was 4.8% and 4.0% respectively will gives significant and maximal OD620 (0.270). It is suggested that in future study, the range of selected prebiotics and probiotics should be diverse, thus experimental data would be more precise and reliable for future references. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109156/1/109156.PDF Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Awang Jalil, Nora
Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil
description Lactobacillus plantarum (L. plantarum) is one of the probiotics that widely studied since the fact that, it has copious potential health benefits. In this study, growth L. plantarum was studied by addition of prebiotics and non prebiotics such as com starch, gum Arabic and glucose. The fermentation of L. plantarum was done in shake flasks and incubated aerobically at 37°C for 24 hours and the result was measured by optical density at 620nm wavelength (OD620) using spectrophotometer. The fermentation media was prepared for 15 runs with different concentrations of prebiotics and non-prebiotic by following three factorial Box-Behnken designs. The principle of this study is to screen prebiotics by investigate the main and interaction effect on the cell growth of L. plantarum. In this experiment, L. plantarum fermentation revealed growth patterns which include lag phase, exponential phase, stationary phase and death phase. Based on the results, maximal optical density of L. plantarum was 0.226 OD620 which contain high proportion of com starch. However, referring to specific growth rate of L. plantarum, it was high when the maximum presence of gum Arabic compared to com starch. For further results, analysis of variance (ANOVA) indicated that all prebiotics and · non-prebiotic had no significance difference (p>0.05) on growth capability of this strain. Response surface methodology (RSM) predicted optimum concentrations of prebiotics, only concentration of gum Arabic and glucose was 4.8% and 4.0% respectively will gives significant and maximal OD620 (0.270). It is suggested that in future study, the range of selected prebiotics and probiotics should be diverse, thus experimental data would be more precise and reliable for future references.
format Thesis
author Awang Jalil, Nora
author_facet Awang Jalil, Nora
author_sort Awang Jalil, Nora
title Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil
title_short Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil
title_full Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil
title_fullStr Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil
title_full_unstemmed Preliminary screening of prebiotics on lactobacillus plantarum L9 growth capability / Nora Awang Jalil
title_sort preliminary screening of prebiotics on lactobacillus plantarum l9 growth capability / nora awang jalil
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109156/1/109156.PDF
https://ir.uitm.edu.my/id/eprint/109156/
_version_ 1825165107404996608
score 13.239859

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