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Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim

IFIH1 is one of the Non-HLA gene that involved in the coding of the immune system. Though not directly involved as the cause of the disease, it was involved in the destruction of the beta-cell of the pancreas due to the altered immune cell system. The method used to detect the allele of interest whi...

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Main Author: Abdul Rahim, Suhaila
Format: Thesis
Language:English
Published: 2013
Subjects:
Pharmacopoeias
Online Access:https://ir.uitm.edu.my/id/eprint/109152/1/109152.PDF
https://ir.uitm.edu.my/id/eprint/109152/
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spelling my.uitm.ir.1091522025-02-21T23:54:48Z https://ir.uitm.edu.my/id/eprint/109152/ Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim Abdul Rahim, Suhaila Pharmacopoeias IFIH1 is one of the Non-HLA gene that involved in the coding of the immune system. Though not directly involved as the cause of the disease, it was involved in the destruction of the beta-cell of the pancreas due to the altered immune cell system. The method used to detect the allele of interest which was rs3741537 in the IF/HJ gene was executed through the Nested PCR method. This study altogether had mainly focusing on the optimization of the Nested PCR method itself in which different parameters which includes concentration of DNA template, annealing temperature, number of cycle and the volume of primers were altered and experimented with. Upon completion of the Nested PCR, the band formation of the gene was evaluated by the gel electrophoresis. In conclusion, the study in developing the optimization of Nested PCR was successful in which the wild type allele and the mutant type allele was able to be differentiated. Thus, from this study, it can be seen that Nested PCR was a method that can be applied for Malaysian in detecting the possible chances of having the type 1 Diabetes and at the same time also being able to be the possible target for type 1 Diabetes treatment. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109152/1/109152.PDF Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim. (2013) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmacopoeias
spellingShingle Pharmacopoeias
Abdul Rahim, Suhaila
Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim
description IFIH1 is one of the Non-HLA gene that involved in the coding of the immune system. Though not directly involved as the cause of the disease, it was involved in the destruction of the beta-cell of the pancreas due to the altered immune cell system. The method used to detect the allele of interest which was rs3741537 in the IF/HJ gene was executed through the Nested PCR method. This study altogether had mainly focusing on the optimization of the Nested PCR method itself in which different parameters which includes concentration of DNA template, annealing temperature, number of cycle and the volume of primers were altered and experimented with. Upon completion of the Nested PCR, the band formation of the gene was evaluated by the gel electrophoresis. In conclusion, the study in developing the optimization of Nested PCR was successful in which the wild type allele and the mutant type allele was able to be differentiated. Thus, from this study, it can be seen that Nested PCR was a method that can be applied for Malaysian in detecting the possible chances of having the type 1 Diabetes and at the same time also being able to be the possible target for type 1 Diabetes treatment.
format Thesis
author Abdul Rahim, Suhaila
author_facet Abdul Rahim, Suhaila
author_sort Abdul Rahim, Suhaila
title Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim
title_short Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim
title_full Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim
title_fullStr Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim
title_full_unstemmed Optimization of PCR method for detection of single nucleotide polymorphism of IFIH1 gene / Suhaila Abdul Rahim
title_sort optimization of pcr method for detection of single nucleotide polymorphism of ifih1 gene / suhaila abdul rahim
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109152/1/109152.PDF
https://ir.uitm.edu.my/id/eprint/109152/
_version_ 1825165107240370176
score 13.239859

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