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Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin

Proteases were extracted from starfruit of different maturity index of Index 2 (light green) and Index 7 (orange). The extracted proteases were purified using acetone and 40% ammo-nium sulphate. The enzyme activity of the obtained proteases were analysed for stability at different pH, temperature an...

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Main Author: Zainuddin, Ezzana Zuraini
Format: Thesis
Language:English
Published: 2013
Subjects:
Bioactive compounds
Online Access:https://ir.uitm.edu.my/id/eprint/109074/3/109074.pdf
https://ir.uitm.edu.my/id/eprint/109074/
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spelling my.uitm.ir.1090742025-01-16T08:29:17Z https://ir.uitm.edu.my/id/eprint/109074/ Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin Zainuddin, Ezzana Zuraini Bioactive compounds Proteases were extracted from starfruit of different maturity index of Index 2 (light green) and Index 7 (orange). The extracted proteases were purified using acetone and 40% ammo-nium sulphate. The enzyme activity of the obtained proteases were analysed for stability at different pH, temperature and during storage at 4°C. Molecular weight distribution of purified proteases were also determined with SDS-PAGE electrophoresis. Protein content of starfruit is 0.73%. Protein concentration of purified protease with 40% ammonium sulphate was higher compared to the acetone purification. Index 2 protease purified with a 40% ammonium sulphate have high enzyme activity at pH 8 and temperature 60°C while for Index 7 at pH 6 and 60°C. Index 2 protease purified with acetone have high enzyme activity at pH 8 and at 50°C while for Index 7 at pH 6 and 50°C. The enzyme activity of both maturity index of protease purified with acetone and 40% ammonium sulphate decreased with storage time. Starfruit protease had high enzyme activity in the range of 100 to 1100 CDU/mg. The protein band at 50 kDa was found in all the prepared proteases. Protein bands were clear for protease purified with acetone compared to the proteases purified with 40% ammonium sulfate. This study suggested that acetone precipitation is a better method to purify starfruit protease and protease content and activity are higher in starfruit at the unripe stage which is Index 2. Starfruit proteases may be used as a protein hydrolysing agent in several areas in food industries. 2013 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/109074/3/109074.pdf Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin. (2013) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/109074.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Bioactive compounds
spellingShingle Bioactive compounds
Zainuddin, Ezzana Zuraini
Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin
description Proteases were extracted from starfruit of different maturity index of Index 2 (light green) and Index 7 (orange). The extracted proteases were purified using acetone and 40% ammo-nium sulphate. The enzyme activity of the obtained proteases were analysed for stability at different pH, temperature and during storage at 4°C. Molecular weight distribution of purified proteases were also determined with SDS-PAGE electrophoresis. Protein content of starfruit is 0.73%. Protein concentration of purified protease with 40% ammonium sulphate was higher compared to the acetone purification. Index 2 protease purified with a 40% ammonium sulphate have high enzyme activity at pH 8 and temperature 60°C while for Index 7 at pH 6 and 60°C. Index 2 protease purified with acetone have high enzyme activity at pH 8 and at 50°C while for Index 7 at pH 6 and 50°C. The enzyme activity of both maturity index of protease purified with acetone and 40% ammonium sulphate decreased with storage time. Starfruit protease had high enzyme activity in the range of 100 to 1100 CDU/mg. The protein band at 50 kDa was found in all the prepared proteases. Protein bands were clear for protease purified with acetone compared to the proteases purified with 40% ammonium sulfate. This study suggested that acetone precipitation is a better method to purify starfruit protease and protease content and activity are higher in starfruit at the unripe stage which is Index 2. Starfruit proteases may be used as a protein hydrolysing agent in several areas in food industries.
format Thesis
author Zainuddin, Ezzana Zuraini
author_facet Zainuddin, Ezzana Zuraini
author_sort Zainuddin, Ezzana Zuraini
title Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin
title_short Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin
title_full Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin
title_fullStr Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin
title_full_unstemmed Extraction of protease from starfruit (Averrhoa carambola L.) / Ezzana Zuraini Zainuddin
title_sort extraction of protease from starfruit (averrhoa carambola l.) / ezzana zuraini zainuddin
publishDate 2013
url https://ir.uitm.edu.my/id/eprint/109074/3/109074.pdf
https://ir.uitm.edu.my/id/eprint/109074/
_version_ 1823097866086776832
score 13.236483

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