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Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham

Resistance of breast cancer to radiotherapy is the crucial aspect leading to relapse and low survival rate. Radioresistance is suggested to be linked with epithelial-mesenchymal transition (EMT), a process involved in regulating cancer tissue remodelling, resulting in recurrence and metastasis. The...

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Main Author: Ronny Sham, Nur Fatihah
Format: Thesis
Language:English
Published: 2024
Subjects:
Medical technology
Cancer
Online Access:https://ir.uitm.edu.my/id/eprint/107652/1/107652.pdf
https://ir.uitm.edu.my/id/eprint/107652/
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spelling my.uitm.ir.1076522024-12-09T08:31:59Z https://ir.uitm.edu.my/id/eprint/107652/ Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham Ronny Sham, Nur Fatihah Medical technology Cancer Resistance of breast cancer to radiotherapy is the crucial aspect leading to relapse and low survival rate. Radioresistance is suggested to be linked with epithelial-mesenchymal transition (EMT), a process involved in regulating cancer tissue remodelling, resulting in recurrence and metastasis. The underlying mechanisms in acquiring radioresistance to gamma-ray in mouse mammary cancer cell lines (EMT6) were investigated using both in vitro and in vivo approaches. EMT6 cells were irradiated with gamma-ray at 2 Gy/cycle to initiate the development of radioresistance in vitro. Confirmation of EMT6 cells acquired radioresistance was analysed using migration and clonogenic assays. Next-generation sequencing analysis validated 16 genes of interest (GOI) via real-time polymerase chain reaction (qPCR). The signalling pathways and proteins involved in EMT6 cells acquiring radioresistance were verified by KEGG pathway analysis and western blotting, respectively. EMT6RR_MJI radioresistance cells were developed from parental EMT6 cells after 8 cycles of fractionated gamma-ray irradiation. This is confirmed by irradiation with gamma-ray at 2, 4, and 8 Gy, which resulted in higher survival fractions and migratory rates of EMT6RR_MJI compared to parental cells. 2024 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/107652/1/107652.pdf Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham. (2024) PhD thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/107652.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Medical technology
Cancer
spellingShingle Medical technology
Cancer
Ronny Sham, Nur Fatihah
Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
description Resistance of breast cancer to radiotherapy is the crucial aspect leading to relapse and low survival rate. Radioresistance is suggested to be linked with epithelial-mesenchymal transition (EMT), a process involved in regulating cancer tissue remodelling, resulting in recurrence and metastasis. The underlying mechanisms in acquiring radioresistance to gamma-ray in mouse mammary cancer cell lines (EMT6) were investigated using both in vitro and in vivo approaches. EMT6 cells were irradiated with gamma-ray at 2 Gy/cycle to initiate the development of radioresistance in vitro. Confirmation of EMT6 cells acquired radioresistance was analysed using migration and clonogenic assays. Next-generation sequencing analysis validated 16 genes of interest (GOI) via real-time polymerase chain reaction (qPCR). The signalling pathways and proteins involved in EMT6 cells acquiring radioresistance were verified by KEGG pathway analysis and western blotting, respectively. EMT6RR_MJI radioresistance cells were developed from parental EMT6 cells after 8 cycles of fractionated gamma-ray irradiation. This is confirmed by irradiation with gamma-ray at 2, 4, and 8 Gy, which resulted in higher survival fractions and migratory rates of EMT6RR_MJI compared to parental cells.
format Thesis
author Ronny Sham, Nur Fatihah
author_facet Ronny Sham, Nur Fatihah
author_sort Ronny Sham, Nur Fatihah
title Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_short Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_full Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_fullStr Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_full_unstemmed Investigation of the pathways involved in the acquired radioresistant EMT6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / Nur Fatihah Ronny Sham
title_sort investigation of the pathways involved in the acquired radioresistant emt6 mouse mammary carcinoma cells to gamma-ray irradiation: in vitro and in vivo studies / nur fatihah ronny sham
publishDate 2024
url https://ir.uitm.edu.my/id/eprint/107652/1/107652.pdf
https://ir.uitm.edu.my/id/eprint/107652/
_version_ 1818838380695257088
score 13.222552

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