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Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim

The aim of this study is to develop a genetic test using specific primers to defect the polymorphism of the Human Epidermal Receptor (HER-2) gene. PCR is used to amplify a specific region of DNA in order to produce a large number of nearly identical copies. The method uses a heat stable DNA replicat...

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Main Author: Ibrahim, Nur Saeidah
Format: Thesis
Language:English
Published: 2009
Subjects:
Pharmaceutical industry
Online Access:https://ir.uitm.edu.my/id/eprint/107051/1/107051.PDF
https://ir.uitm.edu.my/id/eprint/107051/
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spelling my.uitm.ir.1070512024-12-10T23:12:15Z https://ir.uitm.edu.my/id/eprint/107051/ Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim Ibrahim, Nur Saeidah Pharmaceutical industry The aim of this study is to develop a genetic test using specific primers to defect the polymorphism of the Human Epidermal Receptor (HER-2) gene. PCR is used to amplify a specific region of DNA in order to produce a large number of nearly identical copies. The method uses a heat stable DNA replication enzyme called a DNA polymerase, the four deoxynucleotide building blocks of DNA and two small single-stranded DNA segments called primers. Polymerase Chain Reaction using first set of primers was done to amplify the specific sequence of HER-2 gene while the second set of primers was used to amplify the allele specific polymorphism site of HER-2 gene. Both have different fragment size. The PCR products were analyzed using the gel electrophoresis to determine the DNA fragment produced. The bands are observed via gel imaging using the ultra violet (UV) light. The required bands were compared to the standard ladder or marker with known fragment size. Result showed one band indicate homozygous while two bands indicate heterozygous gene. However, for further confirmation, the product that was obtained in this study should be sent for sequencing. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/107051/1/107051.PDF Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim. (2009) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmaceutical industry
spellingShingle Pharmaceutical industry
Ibrahim, Nur Saeidah
Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
description The aim of this study is to develop a genetic test using specific primers to defect the polymorphism of the Human Epidermal Receptor (HER-2) gene. PCR is used to amplify a specific region of DNA in order to produce a large number of nearly identical copies. The method uses a heat stable DNA replication enzyme called a DNA polymerase, the four deoxynucleotide building blocks of DNA and two small single-stranded DNA segments called primers. Polymerase Chain Reaction using first set of primers was done to amplify the specific sequence of HER-2 gene while the second set of primers was used to amplify the allele specific polymorphism site of HER-2 gene. Both have different fragment size. The PCR products were analyzed using the gel electrophoresis to determine the DNA fragment produced. The bands are observed via gel imaging using the ultra violet (UV) light. The required bands were compared to the standard ladder or marker with known fragment size. Result showed one band indicate homozygous while two bands indicate heterozygous gene. However, for further confirmation, the product that was obtained in this study should be sent for sequencing.
format Thesis
author Ibrahim, Nur Saeidah
author_facet Ibrahim, Nur Saeidah
author_sort Ibrahim, Nur Saeidah
title Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_short Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_full Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_fullStr Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_full_unstemmed Development of a genetic test for detection of polymorphism of human epidermal receptor-2 (HER-2) gene / Nur Saeidah Ibrahim
title_sort development of a genetic test for detection of polymorphism of human epidermal receptor-2 (her-2) gene / nur saeidah ibrahim
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/107051/1/107051.PDF
https://ir.uitm.edu.my/id/eprint/107051/
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score 13.223943

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