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Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli

Bisphenol A (BPA) is a xenobiotic extrogenic compound which has been suspected to have estrogenic effects on reproductive system of males and females. This study is conducted to investigate on physical and gene expression changes of male hormones in male SD rats after administration of different dos...

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Main Author: Zulkifli, Shazwani
Format: Thesis
Language:English
Published: 2009
Subjects:
Pharmaceutical industry
Pharmaceutical chemistry
Online Access:https://ir.uitm.edu.my/id/eprint/107028/1/107028.PDF
https://ir.uitm.edu.my/id/eprint/107028/
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spelling my.uitm.ir.1070282024-12-10T23:13:24Z https://ir.uitm.edu.my/id/eprint/107028/ Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli Zulkifli, Shazwani Pharmaceutical industry Pharmaceutical chemistry Bisphenol A (BPA) is a xenobiotic extrogenic compound which has been suspected to have estrogenic effects on reproductive system of males and females. This study is conducted to investigate on physical and gene expression changes of male hormones in male SD rats after administration of different dose of BP A. Six groups of SD male rats (post natal day 28) were used and four groups among them were administered daily orally with 50µg/kg body weight/day, 500µg/kg body weight/day, 1000 µg/kg body weight/day and 5000 µg/kg body weight/day. The other groups were negative control and positive control. In this study, RNA samples have been isolated and amplified using Reverse Transcriptase Polymerase Chain Reaction (RT PCR). Gene expression changes of androgen receptor were examined by using gel electrophoresis method. This study shows no significant difference of body weight and testis changes (P > 0.05) compared to negative control group. Gene expression changes are unsuccessful due to lack of RNA integrity and contamination of the DNA ladder. To improve the study, the samples need to be stored in correct temperature and optimize action of experimental procedure have to be done to reduce risk of contamination. 2009 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/107028/1/107028.PDF Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli. (2009) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Pharmaceutical industry
Pharmaceutical chemistry
spellingShingle Pharmaceutical industry
Pharmaceutical chemistry
Zulkifli, Shazwani
Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli
description Bisphenol A (BPA) is a xenobiotic extrogenic compound which has been suspected to have estrogenic effects on reproductive system of males and females. This study is conducted to investigate on physical and gene expression changes of male hormones in male SD rats after administration of different dose of BP A. Six groups of SD male rats (post natal day 28) were used and four groups among them were administered daily orally with 50µg/kg body weight/day, 500µg/kg body weight/day, 1000 µg/kg body weight/day and 5000 µg/kg body weight/day. The other groups were negative control and positive control. In this study, RNA samples have been isolated and amplified using Reverse Transcriptase Polymerase Chain Reaction (RT PCR). Gene expression changes of androgen receptor were examined by using gel electrophoresis method. This study shows no significant difference of body weight and testis changes (P > 0.05) compared to negative control group. Gene expression changes are unsuccessful due to lack of RNA integrity and contamination of the DNA ladder. To improve the study, the samples need to be stored in correct temperature and optimize action of experimental procedure have to be done to reduce risk of contamination.
format Thesis
author Zulkifli, Shazwani
author_facet Zulkifli, Shazwani
author_sort Zulkifli, Shazwani
title Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli
title_short Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli
title_full Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli
title_fullStr Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli
title_full_unstemmed Gene expression changes in male reproductive system of dose response, BPA-treated sprague dawley rats / Shazwani Zulkifli
title_sort gene expression changes in male reproductive system of dose response, bpa-treated sprague dawley rats / shazwani zulkifli
publishDate 2009
url https://ir.uitm.edu.my/id/eprint/107028/1/107028.PDF
https://ir.uitm.edu.my/id/eprint/107028/
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score 13.222552

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