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Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar

The binary vector is an essential component in Agrobacterium-mediated transformation. It carries the DNA fragments to be delivered into the plant genome. Many binary vectors have been developed for different transformation experiments. Some of the binary vectors are commercially available. Most of t...

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Main Author: Omar, Nawawi
Format: Thesis
Language:English
Published: 2023
Subjects:
Genetics
Physical plant physiology
Online Access:https://ir.uitm.edu.my/id/eprint/106824/1/106824.pdf
https://ir.uitm.edu.my/id/eprint/106824/
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spelling my.uitm.ir.1068242024-12-05T04:41:21Z https://ir.uitm.edu.my/id/eprint/106824/ Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar Omar, Nawawi Genetics Physical plant physiology The binary vector is an essential component in Agrobacterium-mediated transformation. It carries the DNA fragments to be delivered into the plant genome. Many binary vectors have been developed for different transformation experiments. Some of the binary vectors are commercially available. Most of the binary vectors make use of the viral-derived CaMV 35S promoter and the NOS terminator from bacteria to regulate the expression of the transgene. Besides, the binary vector also contains a selectable marker gene for transformant selection. Using viral-derived regulatory elements has posed particular problems in transgenic plants and raised some biosafety concerns. Therefore, a binary vector containing the plant-derived gene regulatory elements is deemed required. In this study, a new binary vector named pJASIN101 was developed using the backbone of the pMDC162 vector and evaluated in model plants through transient transformation experiments. 2023 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/106824/1/106824.pdf Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar. (2023) Masters thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/106824.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Genetics
Physical plant physiology
spellingShingle Genetics
Physical plant physiology
Omar, Nawawi
Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
description The binary vector is an essential component in Agrobacterium-mediated transformation. It carries the DNA fragments to be delivered into the plant genome. Many binary vectors have been developed for different transformation experiments. Some of the binary vectors are commercially available. Most of the binary vectors make use of the viral-derived CaMV 35S promoter and the NOS terminator from bacteria to regulate the expression of the transgene. Besides, the binary vector also contains a selectable marker gene for transformant selection. Using viral-derived regulatory elements has posed particular problems in transgenic plants and raised some biosafety concerns. Therefore, a binary vector containing the plant-derived gene regulatory elements is deemed required. In this study, a new binary vector named pJASIN101 was developed using the backbone of the pMDC162 vector and evaluated in model plants through transient transformation experiments.
format Thesis
author Omar, Nawawi
author_facet Omar, Nawawi
author_sort Omar, Nawawi
title Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
title_short Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
title_full Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
title_fullStr Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
title_full_unstemmed Development and evaluation of a new binary vector for gene expression in higher plants / Nawawi Omar
title_sort development and evaluation of a new binary vector for gene expression in higher plants / nawawi omar
publishDate 2023
url https://ir.uitm.edu.my/id/eprint/106824/1/106824.pdf
https://ir.uitm.edu.my/id/eprint/106824/
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score 13.223943

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