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NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman

This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known....

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Main Author: Rahman, Norsuryahani
Format: Thesis
Language:English
Published: 2008
Subjects:
Organic chemistry
Online Access:https://ir.uitm.edu.my/id/eprint/103906/1/103906.PDF
https://ir.uitm.edu.my/id/eprint/103906/
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spelling my.uitm.ir.1039062024-09-28T23:35:25Z https://ir.uitm.edu.my/id/eprint/103906/ NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman Rahman, Norsuryahani Organic chemistry This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known. Thus, this research project will investigate carbamazepine metabolism by using biological catalyst. Different concentrations of carbamazepine were used with different concentrations of CYP206 enzyme in two differents pH of media. After incubation, the reaction mixtures were extracted using chloroform. The residues were subjected to analysis using chromatographic and spectroscopic technique. The transformed metabolites of carbamazepine were purified through thin layer chromatography (TLC) and structure metabolites were elucidated through nuclear magnetic resonance (NMR) spectroscopy. As a result of carbamazepine metabolism by CYP206 enzyme, the metabolites being produced are 10, 11-dihydroxycarbamazepine and 10, 11-dihydroxy-10-hydroxycarbamazepine. Even though the compounds are not main metabolites in metabolism of carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of carbamazepine. 2008 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/103906/1/103906.PDF NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman. (2008) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Organic chemistry
spellingShingle Organic chemistry
Rahman, Norsuryahani
NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
description This project is aimed to identify metabolites of carbamazepine by action of CYP206 enzyme. Carbamazepine has numbers of side effects. It is believed that metabolites of carbamazepine are responsible for the occurrence of its side effects although the mechanism underlying the reactions is not known. Thus, this research project will investigate carbamazepine metabolism by using biological catalyst. Different concentrations of carbamazepine were used with different concentrations of CYP206 enzyme in two differents pH of media. After incubation, the reaction mixtures were extracted using chloroform. The residues were subjected to analysis using chromatographic and spectroscopic technique. The transformed metabolites of carbamazepine were purified through thin layer chromatography (TLC) and structure metabolites were elucidated through nuclear magnetic resonance (NMR) spectroscopy. As a result of carbamazepine metabolism by CYP206 enzyme, the metabolites being produced are 10, 11-dihydroxycarbamazepine and 10, 11-dihydroxy-10-hydroxycarbamazepine. Even though the compounds are not main metabolites in metabolism of carbamazepine as stated in various literatures, we managed to identify new potential compounds that may have possible contribution in various toxicity problem of carbamazepine.
format Thesis
author Rahman, Norsuryahani
author_facet Rahman, Norsuryahani
author_sort Rahman, Norsuryahani
title NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_short NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_full NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_fullStr NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_full_unstemmed NMR-based identification of carbamazepine metabolism through CYP2D6 enzyme / Norsuryahani Rahman
title_sort nmr-based identification of carbamazepine metabolism through cyp2d6 enzyme / norsuryahani rahman
publishDate 2008
url https://ir.uitm.edu.my/id/eprint/103906/1/103906.PDF
https://ir.uitm.edu.my/id/eprint/103906/
_version_ 1811598327952703488
score 13.211869

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