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Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan

The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved...

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Main Author: Maruan, Khalidah
Format: Thesis
Language:English
Published: 2008
Subjects:
Metabolism
Online Access:https://ir.uitm.edu.my/id/eprint/101907/1/101907.PDF
https://ir.uitm.edu.my/id/eprint/101907/
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spelling my.uitm.ir.1019072024-09-11T19:17:01Z https://ir.uitm.edu.my/id/eprint/101907/ Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan Maruan, Khalidah Metabolism The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved in order to achieve this objective. First process was enzymatic reaction of carbamazepine and followed by extraction and evaporation in order to isolate pure metabolites. The metabolites were further subjected to Thin Layer Chromatography technique for purification and structural characterization by 1H-NMR. Investigation through 1H-NMR identified the metabolic changes in carbamazepine nucleus and the resulting metabolites formed had been identified as 10, 11-dihydro- l 0-hydroxycarbamazepine and I 0, 11- dihydrocarbamazepine. Therefore, with the identification of metabolites formed, this study has accomplished its objective as stated above. 2008 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/101907/1/101907.PDF Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan. (2008) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic Metabolism
spellingShingle Metabolism
Maruan, Khalidah
Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
description The main objective of conducting this study is to identify the metabolites of carbamazepine metabolised by CYP3A4 using various concentrations of carbamazepine and CYP3A4 enzymes at different pH condition and investigate the metabolism through spectroscopic technique. A number of processes involved in order to achieve this objective. First process was enzymatic reaction of carbamazepine and followed by extraction and evaporation in order to isolate pure metabolites. The metabolites were further subjected to Thin Layer Chromatography technique for purification and structural characterization by 1H-NMR. Investigation through 1H-NMR identified the metabolic changes in carbamazepine nucleus and the resulting metabolites formed had been identified as 10, 11-dihydro- l 0-hydroxycarbamazepine and I 0, 11- dihydrocarbamazepine. Therefore, with the identification of metabolites formed, this study has accomplished its objective as stated above.
format Thesis
author Maruan, Khalidah
author_facet Maruan, Khalidah
author_sort Maruan, Khalidah
title Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_short Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_full Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_fullStr Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_full_unstemmed Carbamazepine metabolism by CYP3A4: in vitro studies using NMR spectroscopy / Khalidah Maruan
title_sort carbamazepine metabolism by cyp3a4: in vitro studies using nmr spectroscopy / khalidah maruan
publishDate 2008
url https://ir.uitm.edu.my/id/eprint/101907/1/101907.PDF
https://ir.uitm.edu.my/id/eprint/101907/
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score 13.211869

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