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Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi

The Burton et al. (1985) procedure was selected to identify and separate phospholipids in three marine fishes. The three marine fishes were herring, mackerel and sardine, which were selected among the most consumed fishes in Malaysia. Two different concentrations of SDS were used in the extraction s...

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Main Author: Azizi, Nazathul Shima
Format: Thesis
Language:English
Published: 2003
Online Access:https://ir.uitm.edu.my/id/eprint/101842/1/101842.pdf
https://ir.uitm.edu.my/id/eprint/101842/
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spelling my.uitm.ir.1018422024-09-12T16:29:10Z https://ir.uitm.edu.my/id/eprint/101842/ Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi Azizi, Nazathul Shima The Burton et al. (1985) procedure was selected to identify and separate phospholipids in three marine fishes. The three marine fishes were herring, mackerel and sardine, which were selected among the most consumed fishes in Malaysia. Two different concentrations of SDS were used in the extraction system. The primary objectives were to separate and identify various phospholipids in the fishes as well as to compare the usage of two different SDS concentrations on the extraction system in term of total lipid yield. From this analysis, the most separated and identified phospholipids among the three fishes, were Phosphotidylethanolamine (PE) and Phosphotidycholine (PC). When extracting, it was found out that the lower the concentration of SDS used, it will give higher total lipids. In fish samples, the usage of different concentration in SDS solutions gave different detection of phospholipids. For 0.05M of SDS, PI, PE and PC can be identified in Herring, PE and PC in both Mackerel and Sardine. As comparison, 0.1M of SDS concentration will give the following results; only PE and PC can be detected in Herring, PE, whereas only PE and PC can be detected in both sample Mackerel and Sardine 2003 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/101842/1/101842.pdf Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi. (2003) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/101842.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description The Burton et al. (1985) procedure was selected to identify and separate phospholipids in three marine fishes. The three marine fishes were herring, mackerel and sardine, which were selected among the most consumed fishes in Malaysia. Two different concentrations of SDS were used in the extraction system. The primary objectives were to separate and identify various phospholipids in the fishes as well as to compare the usage of two different SDS concentrations on the extraction system in term of total lipid yield. From this analysis, the most separated and identified phospholipids among the three fishes, were Phosphotidylethanolamine (PE) and Phosphotidycholine (PC). When extracting, it was found out that the lower the concentration of SDS used, it will give higher total lipids. In fish samples, the usage of different concentration in SDS solutions gave different detection of phospholipids. For 0.05M of SDS, PI, PE and PC can be identified in Herring, PE and PC in both Mackerel and Sardine. As comparison, 0.1M of SDS concentration will give the following results; only PE and PC can be detected in Herring, PE, whereas only PE and PC can be detected in both sample Mackerel and Sardine
format Thesis
author Azizi, Nazathul Shima
spellingShingle Azizi, Nazathul Shima
Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi
author_facet Azizi, Nazathul Shima
author_sort Azizi, Nazathul Shima
title Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi
title_short Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi
title_full Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi
title_fullStr Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi
title_full_unstemmed Preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / Nazathul Shima Azizi
title_sort preliminary study on phospholipids in marine fish species by using high-performance liquid chromatography / nazathul shima azizi
publishDate 2003
url https://ir.uitm.edu.my/id/eprint/101842/1/101842.pdf
https://ir.uitm.edu.my/id/eprint/101842/
_version_ 1811598246622003200
score 13.211869

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