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Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman

Gynura procumbens (Lour.), locally known as "sambung nyawa" is annual evergreen shrub that can be found in Southeast Asia. G.procumbens is used to treat many ailments such as inflammation. G.procumbens'' anti-inflammatory and immunomodulatory activity may be utilized for treatmen...

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Main Author: Azman, Putri Amirah
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/101606/1/101606.pdf
https://ir.uitm.edu.my/id/eprint/101606/
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spelling my.uitm.ir.1016062024-09-12T14:51:40Z https://ir.uitm.edu.my/id/eprint/101606/ Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman Azman, Putri Amirah Gynura procumbens (Lour.), locally known as "sambung nyawa" is annual evergreen shrub that can be found in Southeast Asia. G.procumbens is used to treat many ailments such as inflammation. G.procumbens'' anti-inflammatory and immunomodulatory activity may be utilized for treatment of inflammatory disease. The immune system play crucial role in in the pathogenesis of inflammatory disorders which can be treated using drug that have anti-inflammatory and immunostimulant properties. Interferon-gamma (IFN-y) is type II interferon family, secreted by activated T cell and Natural Killer (NK) cells plays role in macrophage activation, inflammation, T helper (Th 1 cell responses) and immunoediting. Cytokine such as Interferon-y is detected using peripheral blood mononuclear cells (PBMC) because PBMC contain lymphocytes that consist of T cell, B cell, NK cells, monocytes and dendritic cells (Miyahira, 2012). Although G.procumbens has known to have anti-inflammatory properties, the expression of Interferon-gamma towards G.procumbens have yet to be fully discovered. Therefore, we would like to determine the expression of Interferon-y in PBMC treated with aqueous extract of G.procumbens using Luminex Human Magnetic Assay. In the present study, we first demonstrated the phytochemical properties of G.procumbens. Then, 12 ml of whole blood is separated using Ficoll density gradient centrifugation to obtain Peripheral blood mononuclear cells (PBMC). PBMC is treated with G.procumbens with addition of CLI 095 and Polymyxin B as inhibitor. The expression of Interferon-y is measured using Luminex assay. The results show that the expression of Interferon-y in PBMC treated with aqueous extract of G.procumbens showed extremely low value in three different concentration and test group. The results for all groups showed extremely low value (<49.259 I>g/ml) of expression towards Interferon-y where statistically analysis were not necessary. In conclusion, we demonstrate the Peripheral Blood Mononuclear Cells (PBMC) treated with aqueous extract of Gprocmbens do not possess the expression of Interferon-gamma (IFN-y) via TLR 4 receptors and MyD88 pathway. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/101606/1/101606.pdf Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman. (2016) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/101606.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description Gynura procumbens (Lour.), locally known as "sambung nyawa" is annual evergreen shrub that can be found in Southeast Asia. G.procumbens is used to treat many ailments such as inflammation. G.procumbens'' anti-inflammatory and immunomodulatory activity may be utilized for treatment of inflammatory disease. The immune system play crucial role in in the pathogenesis of inflammatory disorders which can be treated using drug that have anti-inflammatory and immunostimulant properties. Interferon-gamma (IFN-y) is type II interferon family, secreted by activated T cell and Natural Killer (NK) cells plays role in macrophage activation, inflammation, T helper (Th 1 cell responses) and immunoediting. Cytokine such as Interferon-y is detected using peripheral blood mononuclear cells (PBMC) because PBMC contain lymphocytes that consist of T cell, B cell, NK cells, monocytes and dendritic cells (Miyahira, 2012). Although G.procumbens has known to have anti-inflammatory properties, the expression of Interferon-gamma towards G.procumbens have yet to be fully discovered. Therefore, we would like to determine the expression of Interferon-y in PBMC treated with aqueous extract of G.procumbens using Luminex Human Magnetic Assay. In the present study, we first demonstrated the phytochemical properties of G.procumbens. Then, 12 ml of whole blood is separated using Ficoll density gradient centrifugation to obtain Peripheral blood mononuclear cells (PBMC). PBMC is treated with G.procumbens with addition of CLI 095 and Polymyxin B as inhibitor. The expression of Interferon-y is measured using Luminex assay. The results show that the expression of Interferon-y in PBMC treated with aqueous extract of G.procumbens showed extremely low value in three different concentration and test group. The results for all groups showed extremely low value (<49.259 I>g/ml) of expression towards Interferon-y where statistically analysis were not necessary. In conclusion, we demonstrate the Peripheral Blood Mononuclear Cells (PBMC) treated with aqueous extract of Gprocmbens do not possess the expression of Interferon-gamma (IFN-y) via TLR 4 receptors and MyD88 pathway.
format Thesis
author Azman, Putri Amirah
spellingShingle Azman, Putri Amirah
Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman
author_facet Azman, Putri Amirah
author_sort Azman, Putri Amirah
title Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman
title_short Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman
title_full Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman
title_fullStr Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman
title_full_unstemmed Determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / Putri Amirah Azman
title_sort determination of interferon-gamma in peripheral blood mononuclear cells treated with aqueous extract of gynura procumbens / putri amirah azman
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/101606/1/101606.pdf
https://ir.uitm.edu.my/id/eprint/101606/
_version_ 1811598229705326592
score 13.211869

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