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Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin

Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eru...

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Main Author: Kamaludin, Mohammad Syazwan
Format: Thesis
Language:English
Published: 2016
Online Access:https://ir.uitm.edu.my/id/eprint/101137/1/101137.pdf
https://ir.uitm.edu.my/id/eprint/101137/
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spelling my.uitm.ir.1011372024-08-27T17:13:09Z https://ir.uitm.edu.my/id/eprint/101137/ Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin Kamaludin, Mohammad Syazwan Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eruptive fevers and diabetes mellitus. The following project was conducted to study the determination of Tumor Necrosis Factor – α (TNF – α) level in Peripheral Blood Mononuclear Cells (PBMC) when treated by using G.procumbens. G.procumbens leaf was extracted by aqueous and ethanolic method with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL). Fresh human blood then layered with Ficoll using density gradient separation segregation method to separate the red blood cells and white blood cells. Then at cell culture method, G.procumbens ethanolic and aqueous extraction and PBMC will be added to different comparing group. RPMI used as a media, Polymyxin B as a LPS inhibitor and CLI 0.95 acts as TLR4 inhibitor. The level detection of TNF – α was determined by using Luminex® Magnetic Assay. Even though, comparing with aqueous and ethanolic extraction with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL), there was no exact value in the expression of TNF – α when detection by using Luminex® Magnetic Assay. For that reason, statistical analysis by using SPSS method was unnecessary to be calculated. The result proved that G.procumbens is safe to be consumed by humans because G.procumbens does not show effect on the production of TNF – α because it is the one of the pro-inflammatory cytokines which is important in signaling and promote systemic inflammation. ). In contrast, G.procumbens exhibit in function as TNF – α inhibitor while TNF – α is vice versa. 2016 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/101137/1/101137.pdf Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin. (2016) Degree thesis, thesis, Universiti Teknologi MARA (UiTM). <http://terminalib.uitm.edu.my/101137.pdf>
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
description Gynura procumbens is plant come from family of Asteraceae and is an annual evergreen shrub that grows plenteous in Southest Asia area such as Malaysia, Thailand and Indonesia. G. procumbens is traditionally used for treatment of kidney disease, cancer, rash, constipation, migrains, hypertension, eruptive fevers and diabetes mellitus. The following project was conducted to study the determination of Tumor Necrosis Factor – α (TNF – α) level in Peripheral Blood Mononuclear Cells (PBMC) when treated by using G.procumbens. G.procumbens leaf was extracted by aqueous and ethanolic method with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL). Fresh human blood then layered with Ficoll using density gradient separation segregation method to separate the red blood cells and white blood cells. Then at cell culture method, G.procumbens ethanolic and aqueous extraction and PBMC will be added to different comparing group. RPMI used as a media, Polymyxin B as a LPS inhibitor and CLI 0.95 acts as TLR4 inhibitor. The level detection of TNF – α was determined by using Luminex® Magnetic Assay. Even though, comparing with aqueous and ethanolic extraction with different concentration (50 μg/mL, 200 μg/mL and 800 μg/mL), there was no exact value in the expression of TNF – α when detection by using Luminex® Magnetic Assay. For that reason, statistical analysis by using SPSS method was unnecessary to be calculated. The result proved that G.procumbens is safe to be consumed by humans because G.procumbens does not show effect on the production of TNF – α because it is the one of the pro-inflammatory cytokines which is important in signaling and promote systemic inflammation. ). In contrast, G.procumbens exhibit in function as TNF – α inhibitor while TNF – α is vice versa.
format Thesis
author Kamaludin, Mohammad Syazwan
spellingShingle Kamaludin, Mohammad Syazwan
Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
author_facet Kamaludin, Mohammad Syazwan
author_sort Kamaludin, Mohammad Syazwan
title Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_short Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_full Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_fullStr Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_full_unstemmed Determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / Mohammad Syazwan Kamaludin
title_sort determination of tumor necrosis factor - α level in peripheral blood mononuclear cells treated with gynura procumbens / mohammad syazwan kamaludin
publishDate 2016
url https://ir.uitm.edu.my/id/eprint/101137/1/101137.pdf
https://ir.uitm.edu.my/id/eprint/101137/
_version_ 1808976160118276096
score 13.211869

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