The antimicrobial activity of endophytes extract from mas cotek (ficus deltoidea) / Jasmin Mohamed Ariff
This study was done to explore the antimicrobial activity of endophyte extract of Mas Cotek (Ficus deltoidea). The high demand of endophyte study has brought this study to investigate the antimicrobial property of Mas Cotek endophytes. The design of this study was successfully divided into four majo...
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| Format: | Thesis |
| Language: | English |
| Published: |
2008
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| Subjects: | |
| Online Access: | https://ir.uitm.edu.my/id/eprint/100913/1/100913.PDF https://ir.uitm.edu.my/id/eprint/100913/ |
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| Summary: | This study was done to explore the antimicrobial activity of endophyte extract of Mas Cotek (Ficus deltoidea). The high demand of endophyte study has brought this study to investigate the antimicrobial property of Mas Cotek endophytes. The design of this study was successfully divided into four major steps. The first part involves the subculture of the endophytes of Ficus Deltoidea plant. The endophytes were labeled as SR4, MR3, MRS, MRl I, and BR 12. These endophytes were isolated from the roots of the plant. Then this step is followed by the extraction of the endophyte on the media for two weeks. The next step comprises the antimicrobial activity where the renowned and common technique used for antibacterial susceptibility testing (disk diffusion test) was used to obtain the zone of inhibition of these strains. This antimicrobial activity was tested against selected bacteria and fungi. Remarkably SR4 and MR3 appeared to have a zone of inhibition towards S.aureus. The minimum inhibitory concentration (MIC) for the resulting extract was 1.56 mg/ml for SR4 and 4.17 mg/ml for MR3 respectively. The result obtain suggest that SR4 and MR3 need to obtain a high level of concentration before being able to give off the antimicrobial effect. |
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