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Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin

Biotransformation has been a subject of interest since centuries ago. It can be used to modify a compound into another compound, which may be hard to obtain using chemical synthesis, by using microorganisms such as endophytes. The study is aimed to carry out the fermentation of a given substrate, to...

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Main Author: Mohd Yasin, Syuhaida
Format: Thesis
Language:English
Published: 2008
Subjects:
QD Chemistry
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Online Access:https://ir.uitm.edu.my/id/eprint/100890/1/100890.PDF
https://ir.uitm.edu.my/id/eprint/100890/
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spelling my.uitm.ir.1008902024-08-26T04:58:20Z https://ir.uitm.edu.my/id/eprint/100890/ Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin Mohd Yasin, Syuhaida QD Chemistry RM Therapeutics. Pharmacology RS Pharmacy and materia medica Biotransformation has been a subject of interest since centuries ago. It can be used to modify a compound into another compound, which may be hard to obtain using chemical synthesis, by using microorganisms such as endophytes. The study is aimed to carry out the fermentation of a given substrate, to identify the biotransformation product, as well as to rationalize the chemical modification induced by the endophytes and compare with standard organic chemistry procedure. It was done by culturing endophytes, feeding them with substrates, incubating the culture for one to two weeks, filtering and extracting the mycelium, and lastly analyzing the extract by using Thin Layer Chromatography, Nuclear Magnetic Resonance, and High Performance Liquid Chromatography technique. It was found that biotransformation of (+)-a-longipinene by Burkholderia has taken place in the experiment. In conclusion, the biotransformation of substrate had been successfully done but further study should be done to purify and identify the resulting compound since it could not be done in this study due to time-constraint. 2008 Thesis NonPeerReviewed text en https://ir.uitm.edu.my/id/eprint/100890/1/100890.PDF Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin. (2008) Degree thesis, thesis, Universiti Teknologi MARA (Kampus Puncak Alam).
institution Universiti Teknologi Mara
building Tun Abdul Razak Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Mara
content_source UiTM Institutional Repository
url_provider http://ir.uitm.edu.my/
language English
topic QD Chemistry
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
spellingShingle QD Chemistry
RM Therapeutics. Pharmacology
RS Pharmacy and materia medica
Mohd Yasin, Syuhaida
Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
description Biotransformation has been a subject of interest since centuries ago. It can be used to modify a compound into another compound, which may be hard to obtain using chemical synthesis, by using microorganisms such as endophytes. The study is aimed to carry out the fermentation of a given substrate, to identify the biotransformation product, as well as to rationalize the chemical modification induced by the endophytes and compare with standard organic chemistry procedure. It was done by culturing endophytes, feeding them with substrates, incubating the culture for one to two weeks, filtering and extracting the mycelium, and lastly analyzing the extract by using Thin Layer Chromatography, Nuclear Magnetic Resonance, and High Performance Liquid Chromatography technique. It was found that biotransformation of (+)-a-longipinene by Burkholderia has taken place in the experiment. In conclusion, the biotransformation of substrate had been successfully done but further study should be done to purify and identify the resulting compound since it could not be done in this study due to time-constraint.
format Thesis
author Mohd Yasin, Syuhaida
author_facet Mohd Yasin, Syuhaida
author_sort Mohd Yasin, Syuhaida
title Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
title_short Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
title_full Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
title_fullStr Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
title_full_unstemmed Malaysian endophytes as a tool of chemical modification / Syuhaida Mohd Yasin
title_sort malaysian endophytes as a tool of chemical modification / syuhaida mohd yasin
publishDate 2008
url https://ir.uitm.edu.my/id/eprint/100890/1/100890.PDF
https://ir.uitm.edu.my/id/eprint/100890/
_version_ 1808976133794824192
score 13.211869

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