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Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer

Glioblastoma (GBM), a Grade IV brain tumour, is a well-known radioresistant cancer. To investigate one of the causes of radioresistance, we studied the capacity for potential lethal damage repair (PLDR) of three altered strains of GBM: T98G, U87 and LN18, irradiated with various ions and various lev...

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Main Authors: Chew, Ming Tsuey *, Nisbet, Andrew *, Suzuki, Masao, Matsufuji, Naruhiro, Murakami, Takeshi, Jones, Bleddyn, Bradley, David A
Format: Article
Language:English
Published: Oxford University Press 2018
Subjects:
QC Physics
QH301 Biology
R Medicine (General)
Online Access:http://eprints.sunway.edu.my/939/1/Chew%20Potential%20lethal.pdf
http://eprints.sunway.edu.my/939/
https://academic.oup.com/jrr/advance-article/doi/10.1093/jrr/rry081/5185937?guestAccessKey=41ca9c10-674d-4f5f-890c-454629cba2b4
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spelling my.sunway.eprints.9392019-08-13T04:15:38Z http://eprints.sunway.edu.my/939/ Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer Chew, Ming Tsuey * Nisbet, Andrew * Suzuki, Masao Matsufuji, Naruhiro Murakami, Takeshi Jones, Bleddyn Bradley, David A QC Physics QH301 Biology R Medicine (General) Glioblastoma (GBM), a Grade IV brain tumour, is a well-known radioresistant cancer. To investigate one of the causes of radioresistance, we studied the capacity for potential lethal damage repair (PLDR) of three altered strains of GBM: T98G, U87 and LN18, irradiated with various ions and various levels of linear energy transfer (LET). The GBM cells were exposed to 12C and 28Si ion beams with LETs of 55, 100 and 200 keV/μm, and with X-ray beams of 1.7 keV/μm. Mono-energetic 12C ions and 28Si ions were generated by the Heavy Ion Medical Accelerator at the National Institute of Radiological Science, Chiba, Japan. Clonogenic assays were used to determine cell inactivation. The ability of the cells to repair potential lethal damage was demonstrated by allowing one identical set of irradiated cells to repair for 24 h before subplating. The results show there is definite PLDR with X-rays, some evidence of PLDR at 55 keV/μm, and minimal PLDR at 100 keV/μm. There is no observable PLDR at 200 keV/μm. This is the first study, to the authors’ knowledge, demonstrating the capability of GBM cells to repair potential lethal damage following charged ion irradiations. It is concluded that a GBM’s PLDR is dependent on LET, dose and GBM strain; and the more radioresistant the cell strain, the greater the PLDR. Oxford University Press 2018-09-02 Article PeerReviewed text en cc_by_nc_4 http://eprints.sunway.edu.my/939/1/Chew%20Potential%20lethal.pdf Chew, Ming Tsuey * and Nisbet, Andrew * and Suzuki, Masao and Matsufuji, Naruhiro and Murakami, Takeshi and Jones, Bleddyn and Bradley, David A (2018) Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer. Journal of Radiation Research. pp. 1-10. ISSN 0449-3060 (In Press) https://academic.oup.com/jrr/advance-article/doi/10.1093/jrr/rry081/5185937?guestAccessKey=41ca9c10-674d-4f5f-890c-454629cba2b4 doi:10.1093/jrr/rry081
institution Sunway University
building Sunway Campus Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Sunway University
content_source Sunway Institutional Repository
url_provider http://eprints.sunway.edu.my/
language English
topic QC Physics
QH301 Biology
R Medicine (General)
spellingShingle QC Physics
QH301 Biology
R Medicine (General)
Chew, Ming Tsuey *
Nisbet, Andrew *
Suzuki, Masao
Matsufuji, Naruhiro
Murakami, Takeshi
Jones, Bleddyn
Bradley, David A
Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
description Glioblastoma (GBM), a Grade IV brain tumour, is a well-known radioresistant cancer. To investigate one of the causes of radioresistance, we studied the capacity for potential lethal damage repair (PLDR) of three altered strains of GBM: T98G, U87 and LN18, irradiated with various ions and various levels of linear energy transfer (LET). The GBM cells were exposed to 12C and 28Si ion beams with LETs of 55, 100 and 200 keV/μm, and with X-ray beams of 1.7 keV/μm. Mono-energetic 12C ions and 28Si ions were generated by the Heavy Ion Medical Accelerator at the National Institute of Radiological Science, Chiba, Japan. Clonogenic assays were used to determine cell inactivation. The ability of the cells to repair potential lethal damage was demonstrated by allowing one identical set of irradiated cells to repair for 24 h before subplating. The results show there is definite PLDR with X-rays, some evidence of PLDR at 55 keV/μm, and minimal PLDR at 100 keV/μm. There is no observable PLDR at 200 keV/μm. This is the first study, to the authors’ knowledge, demonstrating the capability of GBM cells to repair potential lethal damage following charged ion irradiations. It is concluded that a GBM’s PLDR is dependent on LET, dose and GBM strain; and the more radioresistant the cell strain, the greater the PLDR.
format Article
author Chew, Ming Tsuey *
Nisbet, Andrew *
Suzuki, Masao
Matsufuji, Naruhiro
Murakami, Takeshi
Jones, Bleddyn
Bradley, David A
author_facet Chew, Ming Tsuey *
Nisbet, Andrew *
Suzuki, Masao
Matsufuji, Naruhiro
Murakami, Takeshi
Jones, Bleddyn
Bradley, David A
author_sort Chew, Ming Tsuey *
title Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
title_short Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
title_full Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
title_fullStr Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
title_full_unstemmed Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
title_sort potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer
publisher Oxford University Press
publishDate 2018
url http://eprints.sunway.edu.my/939/1/Chew%20Potential%20lethal.pdf
http://eprints.sunway.edu.my/939/
https://academic.oup.com/jrr/advance-article/doi/10.1093/jrr/rry081/5185937?guestAccessKey=41ca9c10-674d-4f5f-890c-454629cba2b4
_version_ 1644324438997467136
score 13.211869

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